TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract

H. K. Yang, R. I. Linnoila, N. K. Conrad, M. J. Krasna, S. C. Aisner, B. E. Johnson, M. J. Kelley

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Patients who initially develop an upper aerodigestive tract cancer have an increased risk of second primary cancers. We examined TP53 and RAS mutations and p53 protein in 21 tumors from 10 patients with upper aerodigestive tract cancer who developed a metachronous tumor, to assess the genetic changes that occur in multiple primary tumors from the same individual. Thirteen of 21 (62%) tumors were found to have mis-sense mutations of either TP53 or RAS. Six tumors had TP53 mutations in codons 5 to 8 and 10 tumors from 7 patients had mutations of codons 12 or 13 of K-RAS. Only one patient had concordance of a mutation in 2 tumors; this mutation occurred in K-RAS and was accompanied by discordance of TP53 mutation. Three patients had tumors discordant for both TP53 and PAS mutations. Smoking related tumors had TP53 and RAS mutations which were transversions in 11 (9 G:C to T:A and 2 G:C to C:G) and transitions in 3 (2 G:C to A:T and I A:T to G:C). Tumors not associated with smoking contained only transitions (both G:C to A:T). p53 protein was detected by immunohistochemistry in 7 of 13 (54%) tumors and was concordant in the multiple tumors of 3 patients. Three of the 7 tumors staining for p53 also had TP53 mutations. Thus, genetic alterations are discordant in multiple primary cancers and the pattern of mutations is similar to that found in patients with a single primary tumor, supporting the concept that these cancers arise independently.

Original languageEnglish (US)
Pages (from-to)229-233
Number of pages5
JournalInternational Journal of Cancer
Volume64
Issue number4
DOIs
StatePublished - 1995

Fingerprint

Mutation
Neoplasms
Codon
Smoking
Second Primary Neoplasms
Proteins
Immunohistochemistry
Staining and Labeling

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract. / Yang, H. K.; Linnoila, R. I.; Conrad, N. K.; Krasna, M. J.; Aisner, S. C.; Johnson, B. E.; Kelley, M. J.

In: International Journal of Cancer, Vol. 64, No. 4, 1995, p. 229-233.

Research output: Contribution to journalArticle

Yang, H. K. ; Linnoila, R. I. ; Conrad, N. K. ; Krasna, M. J. ; Aisner, S. C. ; Johnson, B. E. ; Kelley, M. J. / TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract. In: International Journal of Cancer. 1995 ; Vol. 64, No. 4. pp. 229-233.
@article{6f7ec2bed7134b358848feab3ee63839,
title = "TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract",
abstract = "Patients who initially develop an upper aerodigestive tract cancer have an increased risk of second primary cancers. We examined TP53 and RAS mutations and p53 protein in 21 tumors from 10 patients with upper aerodigestive tract cancer who developed a metachronous tumor, to assess the genetic changes that occur in multiple primary tumors from the same individual. Thirteen of 21 (62{\%}) tumors were found to have mis-sense mutations of either TP53 or RAS. Six tumors had TP53 mutations in codons 5 to 8 and 10 tumors from 7 patients had mutations of codons 12 or 13 of K-RAS. Only one patient had concordance of a mutation in 2 tumors; this mutation occurred in K-RAS and was accompanied by discordance of TP53 mutation. Three patients had tumors discordant for both TP53 and PAS mutations. Smoking related tumors had TP53 and RAS mutations which were transversions in 11 (9 G:C to T:A and 2 G:C to C:G) and transitions in 3 (2 G:C to A:T and I A:T to G:C). Tumors not associated with smoking contained only transitions (both G:C to A:T). p53 protein was detected by immunohistochemistry in 7 of 13 (54{\%}) tumors and was concordant in the multiple tumors of 3 patients. Three of the 7 tumors staining for p53 also had TP53 mutations. Thus, genetic alterations are discordant in multiple primary cancers and the pattern of mutations is similar to that found in patients with a single primary tumor, supporting the concept that these cancers arise independently.",
author = "Yang, {H. K.} and Linnoila, {R. I.} and Conrad, {N. K.} and Krasna, {M. J.} and Aisner, {S. C.} and Johnson, {B. E.} and Kelley, {M. J.}",
year = "1995",
doi = "10.1002/ijc.2910640403",
language = "English (US)",
volume = "64",
pages = "229--233",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - TP53 and RAS mutations in metachronous tumors from patients with cancer of the upper aerodigestive tract

AU - Yang, H. K.

AU - Linnoila, R. I.

AU - Conrad, N. K.

AU - Krasna, M. J.

AU - Aisner, S. C.

AU - Johnson, B. E.

AU - Kelley, M. J.

PY - 1995

Y1 - 1995

N2 - Patients who initially develop an upper aerodigestive tract cancer have an increased risk of second primary cancers. We examined TP53 and RAS mutations and p53 protein in 21 tumors from 10 patients with upper aerodigestive tract cancer who developed a metachronous tumor, to assess the genetic changes that occur in multiple primary tumors from the same individual. Thirteen of 21 (62%) tumors were found to have mis-sense mutations of either TP53 or RAS. Six tumors had TP53 mutations in codons 5 to 8 and 10 tumors from 7 patients had mutations of codons 12 or 13 of K-RAS. Only one patient had concordance of a mutation in 2 tumors; this mutation occurred in K-RAS and was accompanied by discordance of TP53 mutation. Three patients had tumors discordant for both TP53 and PAS mutations. Smoking related tumors had TP53 and RAS mutations which were transversions in 11 (9 G:C to T:A and 2 G:C to C:G) and transitions in 3 (2 G:C to A:T and I A:T to G:C). Tumors not associated with smoking contained only transitions (both G:C to A:T). p53 protein was detected by immunohistochemistry in 7 of 13 (54%) tumors and was concordant in the multiple tumors of 3 patients. Three of the 7 tumors staining for p53 also had TP53 mutations. Thus, genetic alterations are discordant in multiple primary cancers and the pattern of mutations is similar to that found in patients with a single primary tumor, supporting the concept that these cancers arise independently.

AB - Patients who initially develop an upper aerodigestive tract cancer have an increased risk of second primary cancers. We examined TP53 and RAS mutations and p53 protein in 21 tumors from 10 patients with upper aerodigestive tract cancer who developed a metachronous tumor, to assess the genetic changes that occur in multiple primary tumors from the same individual. Thirteen of 21 (62%) tumors were found to have mis-sense mutations of either TP53 or RAS. Six tumors had TP53 mutations in codons 5 to 8 and 10 tumors from 7 patients had mutations of codons 12 or 13 of K-RAS. Only one patient had concordance of a mutation in 2 tumors; this mutation occurred in K-RAS and was accompanied by discordance of TP53 mutation. Three patients had tumors discordant for both TP53 and PAS mutations. Smoking related tumors had TP53 and RAS mutations which were transversions in 11 (9 G:C to T:A and 2 G:C to C:G) and transitions in 3 (2 G:C to A:T and I A:T to G:C). Tumors not associated with smoking contained only transitions (both G:C to A:T). p53 protein was detected by immunohistochemistry in 7 of 13 (54%) tumors and was concordant in the multiple tumors of 3 patients. Three of the 7 tumors staining for p53 also had TP53 mutations. Thus, genetic alterations are discordant in multiple primary cancers and the pattern of mutations is similar to that found in patients with a single primary tumor, supporting the concept that these cancers arise independently.

UR - http://www.scopus.com/inward/record.url?scp=0028978781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028978781&partnerID=8YFLogxK

U2 - 10.1002/ijc.2910640403

DO - 10.1002/ijc.2910640403

M3 - Article

C2 - 7657384

AN - SCOPUS:0028978781

VL - 64

SP - 229

EP - 233

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -