TY - JOUR
T1 - Tracking the Progression of Osteolytic and Osteosclerotic Lesions in Mice Using Serial In Vivo μCT
T2 - Applications to the Assessment of Bisphosphonate Treatment Efficacy
AU - Campbell, Graeme M.
AU - Tower, Robert J.
AU - Damm, Timo
AU - Kneissl, Philipp
AU - Rambow, Anna C.
AU - Schem, Christian
AU - Tiwari, Sanjay
AU - Glüer, Claus C.
N1 - Funding Information:
This work was supported by the Deutsche Forschungsgemein-schaft (DFG) through the Forschergruppe 1586 SKELMET and by the research grant from the state of Schleswig-Holstein and the European Union ERDF-European Regional Development Fund (MOIN CC, Zukunftsprogramm Wirtschaft).
Funding Information:
This work was supported by the Deutsche Forschungsgemeinschaft (DFG) through the Forschergruppe 1586 SKELMET and by the research grant from the state of Schleswig-Holstein and the European Union ERDF-European Regional Development Fund (MOIN CC, Zukunftsprogramm Wirtschaft). Authors? roles: Study design: GMC, RT, CS, ST, and CCG. Study conduct: GMC, RT, TD, PK, ACR, and ST. Data collection: GMC, RT, TD, ACR, and PK. Data analysis: GMC. Data interpretation: GMC, RT, TD, PK, ACR, CS, ST, and CCG. Drafting manuscript: GMC. Revising manuscript content: GMC, RT, TD, PK, ACR, CS, ST, and CCG. Approval of final version of manuscript: GMC, RT, TD, PK, ACR, CS, ST, and CCG. GMC takes responsibility for the integrity of the data analysis.
Publisher Copyright:
© 2017 American Society for Bone and Mineral Research
PY - 2018/3
Y1 - 2018/3
N2 - The metastasis of tumor cells to bone can lead to osteolytic and osteosclerotic lesions, which cause severe, highly-localized bone destruction and abnormal bone apposition, respectively. Accurate quantification of lesion progression is critical to understand underlying mechanisms and assess treatment efficacy; however, standard structural parameters may be insensitive to local changes. We developed methods to quantify osteolytic and osteosclerotic lesions using micro–computed tomography (μCT) within in vivo mouse datasets. Two Balb/c nude datasets were used: (i) bone-homing MDA-MB-231 (osteolytic) cells injected into the left ventricle, treatment with alendronate or vehicle, and weekly μCT (proximal tibia) for 4 weeks, and (ii) MCF7 (osteosclerotic) cells injected into the right tibia and weekly μCT over 12 weeks. After registering images to baseline, osteolytic lesion volume was determined by summing all baseline bone voxels at distances greater than a threshold (150 μm) from the nearest follow-up. Osteosclerotic lesions were determined by measuring the distance from each follow-up surface voxel to the nearest baseline surface and calculating the standard deviation of distance values (SDDT) of the surrounding voxels. Bone mineral density (BMD), bone volume density (BV/TV), and separation (Sp) were determined for comparison. Osteolytic lesions were observed 1 week after tumor cell injection; however, no corresponding BV/TV losses or Sp increases were observed, indicating that standard parameters were unable to detect early metastatic changes. Lesion volume was smaller in the alendronate versus control group (15.0%, p = 0.004 and 18.6%, p = 0.002 of control lesion volume at weeks 3 and 4, respectively). In the osteosclerotic dataset, increased SDDT was observed following injection, providing a potential new measure of osteosclerotic bone apposition. These data show that quantification of local structural change with serial μCT may overcome the limitations of standard mineral and microstructural parameters, and successfully separates metastatic and normal bone turnover.
AB - The metastasis of tumor cells to bone can lead to osteolytic and osteosclerotic lesions, which cause severe, highly-localized bone destruction and abnormal bone apposition, respectively. Accurate quantification of lesion progression is critical to understand underlying mechanisms and assess treatment efficacy; however, standard structural parameters may be insensitive to local changes. We developed methods to quantify osteolytic and osteosclerotic lesions using micro–computed tomography (μCT) within in vivo mouse datasets. Two Balb/c nude datasets were used: (i) bone-homing MDA-MB-231 (osteolytic) cells injected into the left ventricle, treatment with alendronate or vehicle, and weekly μCT (proximal tibia) for 4 weeks, and (ii) MCF7 (osteosclerotic) cells injected into the right tibia and weekly μCT over 12 weeks. After registering images to baseline, osteolytic lesion volume was determined by summing all baseline bone voxels at distances greater than a threshold (150 μm) from the nearest follow-up. Osteosclerotic lesions were determined by measuring the distance from each follow-up surface voxel to the nearest baseline surface and calculating the standard deviation of distance values (SDDT) of the surrounding voxels. Bone mineral density (BMD), bone volume density (BV/TV), and separation (Sp) were determined for comparison. Osteolytic lesions were observed 1 week after tumor cell injection; however, no corresponding BV/TV losses or Sp increases were observed, indicating that standard parameters were unable to detect early metastatic changes. Lesion volume was smaller in the alendronate versus control group (15.0%, p = 0.004 and 18.6%, p = 0.002 of control lesion volume at weeks 3 and 4, respectively). In the osteosclerotic dataset, increased SDDT was observed following injection, providing a potential new measure of osteosclerotic bone apposition. These data show that quantification of local structural change with serial μCT may overcome the limitations of standard mineral and microstructural parameters, and successfully separates metastatic and normal bone turnover.
KW - IMAGE REGISTRATION
KW - MOUSE
KW - OSTEOLYTIC LESION
KW - OSTEOSCLEROTIC LESION
KW - SERIAL µCT
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U2 - 10.1002/jbmr.3317
DO - 10.1002/jbmr.3317
M3 - Article
C2 - 29044710
AN - SCOPUS:85034263695
SN - 0884-0431
VL - 33
SP - 410
EP - 418
JO - Journal of Bone and Mineral Research
JF - Journal of Bone and Mineral Research
IS - 3
ER -