Abstract
We present a reconstruction algorithm that resolves cellular tractions in diffraction-limited nascent adhesions (NAs). The enabling method is the introduction of sparsity regularization to the solution of the inverse problem, which suppresses noise without underestimating traction magnitude. We show that NAs transmit a distinguishable amount of traction and that NA maturation depends on traction growth rate. A software package implementing this numerical approach is provided.
Original language | English (US) |
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Pages (from-to) | 653-656 |
Number of pages | 4 |
Journal | Nature methods |
Volume | 12 |
Issue number | 7 |
DOIs | |
State | Published - Jun 30 2015 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology