Transactivation by Rtg1p, a basic helix-loop-helix protein that functions in communication between mitochondria and the nucleus in yeast

B. A. Rothermel, A. W. Shyjan, J. L. Etheredge, R. A. Butow

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Rtg1p is a basic helix-loop-helix transcription factor in the yeast Saccharomyces cerevisiae that is required for basal and regulated expression of CIT2, the gene encoding a peroxisomal isoform of citrate synthase. In respiratory incompetent ρ° petite cells, CIT2 transcription is elevated as much as 30-fold compared with respiratory competent ρ+ cells. Here we provide evidence that Rtg1p interacts directly with a CIT2 upstream activation site (UAS(r)) and that the ρ°/ρ+ regulation is not due to a change in the levels of Rtg1p. A fusion protein consisting of the DNA binding domain of Gal4p fused to the NH2 terminus of the full-length wild-type Rtg1p was able to transactivate an integrated Latz reporter under control of the Gal4p-responsive GAL1 UAS(G) in a ρ°/ρ+dependent manner. Other Gal4p fusions to deletions or mutations of Rtg1p indicate that the helix-loop- helix domain is essential for transactivation. Regulated expression of CIT2 also requires the RTG2 gene product. The Gal4-Rtg1p fusion was unable to transactivate the Latz reporter gene in a strain deleted for RTG2, suggesting that the RTG2 product does not act independently of Rtg1p in the ρ°/ρ+ transcriptional response.

Original languageEnglish (US)
Pages (from-to)29476-29482
Number of pages7
JournalJournal of Biological Chemistry
Volume270
Issue number49
DOIs
StatePublished - 1995

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Mitochondria
Yeast
Transcriptional Activation
Helix-Loop-Helix Motifs
Fusion reactions
Yeasts
Basic Helix-Loop-Helix Transcription Factors
Citrate (si)-Synthase
Sequence Deletion
Communication
DNA-Binding Proteins
Genes
Reporter Genes
Saccharomyces cerevisiae
Protein Isoforms
Proteins
Gene encoding
Transcription
Gene Expression
Chemical activation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transactivation by Rtg1p, a basic helix-loop-helix protein that functions in communication between mitochondria and the nucleus in yeast. / Rothermel, B. A.; Shyjan, A. W.; Etheredge, J. L.; Butow, R. A.

In: Journal of Biological Chemistry, Vol. 270, No. 49, 1995, p. 29476-29482.

Research output: Contribution to journalArticle

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AU - Butow, R. A.

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