TY - JOUR
T1 - Transcription activation by a PNA-Peptide chimera in a mammalian cell extract
AU - Liu, Bo
AU - Han, Ying
AU - Ferdous, Anwarul
AU - Corey, David R.
AU - Kodadek, Thomas
N1 - Funding Information:
This work was funded by a grant from the NIDDK/NIH (P01-DK58398).
PY - 2003/10
Y1 - 2003/10
N2 - Synthetic activators that mimic the ability of native transcription factors to recruit the RNA polymerase holoenzyme to specific promoters could, in principle, be constructed by joining a sequence-specific DNA binding moiety with a molecule able to bind the holoenzyme. We report here that a peptide nucleic acid (PNA)-peptide chimera is capable of activating transcription in vitro in a HeLa nuclear extract. Specifically, a promoter-targeted PNA alone acts as a strong inhibitor of basal transcription in a HeLa nuclear extract, presumably due to structural modification of the promoter. However, the fusion of a Gal80-binding peptide to the PNA, but not control peptides, reactivates transcription. The Gal80-binding peptide was selected solely on the basis of its ability to bind the yeast repressor.
AB - Synthetic activators that mimic the ability of native transcription factors to recruit the RNA polymerase holoenzyme to specific promoters could, in principle, be constructed by joining a sequence-specific DNA binding moiety with a molecule able to bind the holoenzyme. We report here that a peptide nucleic acid (PNA)-peptide chimera is capable of activating transcription in vitro in a HeLa nuclear extract. Specifically, a promoter-targeted PNA alone acts as a strong inhibitor of basal transcription in a HeLa nuclear extract, presumably due to structural modification of the promoter. However, the fusion of a Gal80-binding peptide to the PNA, but not control peptides, reactivates transcription. The Gal80-binding peptide was selected solely on the basis of its ability to bind the yeast repressor.
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U2 - 10.1016/j.chembiol.2003.09.008
DO - 10.1016/j.chembiol.2003.09.008
M3 - Article
C2 - 14583257
AN - SCOPUS:0142198529
SN - 1074-5521
VL - 10
SP - 909
EP - 916
JO - Chemistry and Biology
JF - Chemistry and Biology
IS - 10
ER -