Abstract
The ability for the eukaryotic cell to transcriptionally respond to various stimuli is critical for the overall homeostasis of the cell, and in turn, the organism. The human RNA polymerase II complex (Pol II), which is responsible for the transcription of protein-encoding genes and non-coding RNAs, is paused at promoter-proximal regions to ensure their rapid activation. In response to stimulation, Pol II pause release is facilitated by the action of positive transcription elongation factors such as the P-TEFb kinase. However, the majority of P-TEFb is held in a catalytically inactivate state, assembled into the 7SK small nuclear ribonucleoprotein (snRNP) complex, and must be dislodged to become catalytically active. In this review, we discuss mechanisms of 7SK snRNP recruitment to promoter-proximal regions and P-TEFb disassembly from the inhibitory snRNP to regulate ‘on site' kinase activation and Pol II pause release.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2115-2123 |
| Number of pages | 9 |
| Journal | Cell Cycle |
| Volume | 15 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 17 2016 |
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Keywords
- 7SK snRNP
- KAP1
- P-TEFb
- RNA polymerase II
- transcription elongation
ASJC Scopus subject areas
- Medicine(all)
- Molecular Biology
- Developmental Biology
- Cell Biology
Cite this
Transcription elongation control by the 7SK snRNP complex : Releasing the pause. / McNamara, Ryan P.; Bacon, Curtis W.; D'Orso, Iván.
In: Cell Cycle, Vol. 15, No. 16, 17.08.2016, p. 2115-2123.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Transcription elongation control by the 7SK snRNP complex
T2 - Releasing the pause
AU - McNamara, Ryan P.
AU - Bacon, Curtis W.
AU - D'Orso, Iván
PY - 2016/8/17
Y1 - 2016/8/17
N2 - The ability for the eukaryotic cell to transcriptionally respond to various stimuli is critical for the overall homeostasis of the cell, and in turn, the organism. The human RNA polymerase II complex (Pol II), which is responsible for the transcription of protein-encoding genes and non-coding RNAs, is paused at promoter-proximal regions to ensure their rapid activation. In response to stimulation, Pol II pause release is facilitated by the action of positive transcription elongation factors such as the P-TEFb kinase. However, the majority of P-TEFb is held in a catalytically inactivate state, assembled into the 7SK small nuclear ribonucleoprotein (snRNP) complex, and must be dislodged to become catalytically active. In this review, we discuss mechanisms of 7SK snRNP recruitment to promoter-proximal regions and P-TEFb disassembly from the inhibitory snRNP to regulate ‘on site' kinase activation and Pol II pause release.
AB - The ability for the eukaryotic cell to transcriptionally respond to various stimuli is critical for the overall homeostasis of the cell, and in turn, the organism. The human RNA polymerase II complex (Pol II), which is responsible for the transcription of protein-encoding genes and non-coding RNAs, is paused at promoter-proximal regions to ensure their rapid activation. In response to stimulation, Pol II pause release is facilitated by the action of positive transcription elongation factors such as the P-TEFb kinase. However, the majority of P-TEFb is held in a catalytically inactivate state, assembled into the 7SK small nuclear ribonucleoprotein (snRNP) complex, and must be dislodged to become catalytically active. In this review, we discuss mechanisms of 7SK snRNP recruitment to promoter-proximal regions and P-TEFb disassembly from the inhibitory snRNP to regulate ‘on site' kinase activation and Pol II pause release.
KW - 7SK snRNP
KW - KAP1
KW - P-TEFb
KW - RNA polymerase II
KW - transcription elongation
UR - http://www.scopus.com/inward/record.url?scp=84982195937&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84982195937&partnerID=8YFLogxK
U2 - 10.1080/15384101.2016.1181241
DO - 10.1080/15384101.2016.1181241
M3 - Review article
C2 - 27152730
AN - SCOPUS:84982195937
VL - 15
SP - 2115
EP - 2123
JO - Cell Cycle
JF - Cell Cycle
SN - 1538-4101
IS - 16
ER -