Transcription factor B cell lineage-specific activator protein regulates the gene for human X-box binding protein 1

Andreas M. Reimold, Paul D. Ponath, Yue Sheng Li, Richard R. Hardy, Chella S. David, Jack L. Strominger, Laurie H. Glimcher

Research output: Contribution to journalArticle

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Abstract

The transcription factor human X-box binding protein 1 (hXBP-1) is a basic region-leucine zipper protein implicated in this regulation of major histocompatibility complex class II gene expression as well as in exocrine gland and skeletal development. Multiple regulatory elements in the hXBP-1 promoter lie 3' to the transcription start site, including the hX2 site, whose core sequence is an AP-1-like element identical to the hXBP-1 target sequence in the HLA-DRA promoter. One complex identified by electrophoretic mobility shift assay (EMSA), complex 3, was previously shown to protect the hX2 site and more 3' bases. Sequence analysis now shows that this region contains a consensus binding site for transcription factor BSAP (B cell lineage-specific activator protein). Complex 3 and BSAP have identical cell- type specificities, as they are found only in pre-B and mature B cell lines. In EMSAs, BSAP antibody specifically recognized complex 3, and in vitro translated BSAP could bind to an hXBP promoter fragment. Cotransfections using an hXBP-1 reporter construct indicated that BSAP downregulates the hXBP-1 promoter. The highest levels of hXBP-1 mRNA were found when BSAP was not expressed, in pre-Pro-B cell and in plasma cell lines. In addition, hXBP-1 and BSAP levels were inversely correlated along the early stages of B cell development. In the regulation of the hXBP-1 promoter, a strong positive transcriptional influence at the hX2 site is opposed by the downregulatory actions of BSAP.

Original languageEnglish (US)
Pages (from-to)393-401
Number of pages9
JournalJournal of Experimental Medicine
Volume183
Issue number2
DOIs
StatePublished - Feb 1 1996

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PAX5 Transcription Factor
Cell Lineage
Transcription Factors
Genes
B-Lymphoid Precursor Cells
HLA-DR alpha-Chains
B-Lymphocytes
Exocrine Glands
Cell Line
Leucine Zippers
MHC Class II Genes
human XBP1 protein
Transcription Initiation Site
Transcription Factor AP-1
Electrophoretic Mobility Shift Assay
Plasma Cells
Major Histocompatibility Complex
Sequence Analysis
Down-Regulation
Binding Sites

ASJC Scopus subject areas

  • Immunology

Cite this

Transcription factor B cell lineage-specific activator protein regulates the gene for human X-box binding protein 1. / Reimold, Andreas M.; Ponath, Paul D.; Li, Yue Sheng; Hardy, Richard R.; David, Chella S.; Strominger, Jack L.; Glimcher, Laurie H.

In: Journal of Experimental Medicine, Vol. 183, No. 2, 01.02.1996, p. 393-401.

Research output: Contribution to journalArticle

Reimold, Andreas M. ; Ponath, Paul D. ; Li, Yue Sheng ; Hardy, Richard R. ; David, Chella S. ; Strominger, Jack L. ; Glimcher, Laurie H. / Transcription factor B cell lineage-specific activator protein regulates the gene for human X-box binding protein 1. In: Journal of Experimental Medicine. 1996 ; Vol. 183, No. 2. pp. 393-401.
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