Transcription factor interactions

Selectors of positive or negative regulation from a single DNA element

Marc I. Diamond, Jeffrey N. Miner, Steven K. Yoshinaga, Keith R. Yamamoto

Research output: Contribution to journalArticle

1019 Citations (Scopus)

Abstract

The mechanism by which a single factor evokes opposite regulatory effects from a specific DNA sequence is not well understood. In this study, a 25-base pair element that resides upstream of the mouse proliferin gene was examined; it conferred on linked promoters either positive or negative glucocorticoid regulation, depending upon physiological context. This sequence, denoted a "composite" glucocorticoid response element (GRE), was bound selectively in vitro both by the glucocorticoid receptor and by c-Jun and c-Fos, components of the. phorbol ester-activated AP-1 transcription factor. Indeed, c-Jun and c-Fos served as selectors of hormone responsiveness: the composite GRE was inactive in the absence of c-Jun, whereas it conferred a positive glucocorticoid effect in the presence of c-Jun, and a negative glucocorticoid effect in the presence of c-Jun and relatively high levels of c-Fos. The receptor also interacted selectively with c-Jun in vitro. A general model for composite GRE action is proposed that invokes both DNA binding and protein-protein interactions by receptor and nonreceptor factors.

Original languageEnglish (US)
Pages (from-to)1266-1272
Number of pages7
JournalScience
Volume249
Issue number4974
StatePublished - Sep 14 1990

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Glucocorticoids
Transcription Factors
DNA
Response Elements
Glucocorticoid Receptors
Transcription Factor AP-1
DNA-Binding Proteins
Phorbol Esters
Base Pairing
Hormones
Genes
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Diamond, M. I., Miner, J. N., Yoshinaga, S. K., & Yamamoto, K. R. (1990). Transcription factor interactions: Selectors of positive or negative regulation from a single DNA element. Science, 249(4974), 1266-1272.

Transcription factor interactions : Selectors of positive or negative regulation from a single DNA element. / Diamond, Marc I.; Miner, Jeffrey N.; Yoshinaga, Steven K.; Yamamoto, Keith R.

In: Science, Vol. 249, No. 4974, 14.09.1990, p. 1266-1272.

Research output: Contribution to journalArticle

Diamond, MI, Miner, JN, Yoshinaga, SK & Yamamoto, KR 1990, 'Transcription factor interactions: Selectors of positive or negative regulation from a single DNA element', Science, vol. 249, no. 4974, pp. 1266-1272.
Diamond, Marc I. ; Miner, Jeffrey N. ; Yoshinaga, Steven K. ; Yamamoto, Keith R. / Transcription factor interactions : Selectors of positive or negative regulation from a single DNA element. In: Science. 1990 ; Vol. 249, No. 4974. pp. 1266-1272.
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