Transcription factors are required for the meiotic recombination hotspot at the HIS4 locus in Saccharomyces cerevisiae

Michael A. White, Margaret Dominska, Thomas D. Petes

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

The full activity of a recombination initiation site located 5′ of HIS4 requires the binding of the transcription factors RAP1, BAS1, and BAS2. Two RAP1 binding sites can substitute for the wild-type initiation site. A 51-bp region of telomeric DNA inserted upstream of either HIS4 or ARG4 very strongly stimulates recombination. We suggest that the ability of transcription factors to induce recombination is a consequence of an altered chromatin structure that favors the entry of proteins that initiate recombination, rather than an effect of these factors on transcription.

Original languageEnglish (US)
Pages (from-to)6621-6625
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number14
DOIs
StatePublished - Jul 15 1993

Keywords

  • Chromatin
  • Heteroduplex
  • Telomere

ASJC Scopus subject areas

  • General

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