Transcription-induced nucleosome 'splitting': An underlying structure for DNase I sensitive chromatin

Myeong Sok Lee, William T. Garrard

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

Utilizing yeast strains containing promoter mutations, we demonstrate that transcription of the HSP82 gene causes nucleosomes toward the 3'-end to become DNase I sensitive and 'split' into structures that exhibit a 'half-nucleosomal' cleavage periodicity. Splitting occurs even when only a few RNA polymerase II molecules are engaged in basal level transcription or during the first round of induced transcription. The split nucleosomal structure survives nuclear isolation suggesting that it may be stabilized by post-translational modifications or non-histone proteins, and may require DNA replication for reversal to a whole nucleosomal structure. Split nucleosomes represent a structure for DNase I sensitive chromatin and are probably of common occurrence but difficult to detect experimentally. We suggest that transient positive supercoils downstream of traversing RNA polymerase lead to nucleosome splitting.

Original languageEnglish (US)
Pages (from-to)607-615
Number of pages9
JournalEMBO Journal
Volume10
Issue number3
DOIs
StatePublished - 1991

Keywords

  • DNase I sensitivity
  • chromatin
  • gene regulation
  • heat shock
  • nucleosome

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Transcription-induced nucleosome 'splitting': An underlying structure for DNase I sensitive chromatin'. Together they form a unique fingerprint.

  • Cite this