Transcription-induced nucleosome 'splitting': An underlying structure for DNase I sensitive chromatin

Myeong Sok Lee, William T. Garrard

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Utilizing yeast strains containing promoter mutations, we demonstrate that transcription of the HSP82 gene causes nucleosomes toward the 3′-end to become DNase I sensitive and 'split' into structures that exhibit a 'half-nucleosomal' cleavage periodicity. Splitting occurs even when only a few RNA polymerase II molecules are engaged in basal level transcription or during the first round of induced transcription. The split nucleosomal structure survives nuclear isolation suggesting that it may be stabilized by post-translational modifications or non-histone proteins, and may require DNA replication for reversal to a whole nucleosomal structure. Split nucleosomes represent a structure for DNase I sensitive chromatin and are probably of common occurrence but difficult to detect experimentally. We suggest that transient positive supercoils downstream of traversing RNA polymerase lead to nucleosome splitting. chromatin/DNase I sensitivity/gene regulation/ heat shock/nucleosome.

Original languageEnglish (US)
Pages (from-to)607-615
Number of pages9
JournalEMBO Journal
Volume10
Issue number3
StatePublished - 1991

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Nucleosomes
Deoxyribonuclease I
Transcription
Chromatin
RNA Polymerase II
Periodicity
DNA-Directed RNA Polymerases
Post Translational Protein Processing
DNA Replication
Gene expression
Yeast
Genes
Shock
Hot Temperature
Yeasts
Mutation
Molecules
DNA
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Transcription-induced nucleosome 'splitting' : An underlying structure for DNase I sensitive chromatin. / Lee, Myeong Sok; Garrard, William T.

In: EMBO Journal, Vol. 10, No. 3, 1991, p. 607-615.

Research output: Contribution to journalArticle

Lee, Myeong Sok ; Garrard, William T. / Transcription-induced nucleosome 'splitting' : An underlying structure for DNase I sensitive chromatin. In: EMBO Journal. 1991 ; Vol. 10, No. 3. pp. 607-615.
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