Using a transcription system derived from rat liver, we have investigated the function of the TATA-box and cap site/initiator regions in assembly of stable intermediates on the pathway leading to formation of a functional RNA polymerase II preinitiation complex. Our findings argue that neither the TATA-box region nor the cap site/initiator region functions independently in the binding of transcription factors during formation of these intermediates, but, rather, the two sequence elements function cooperatively to direct assembly of stable preinitiation complex at the promoter.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Biological Chemistry|
|State||Published - 1990|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology