Transcription of DNA injected into Xenopus oocytes is influenced by template topology

R. M. Harland, H. Weintraub, S. L. McKnight

Research output: Contribution to journalArticle

121 Scopus citations

Abstract

We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.

Original languageEnglish (US)
Pages (from-to)38-43
Number of pages6
JournalNature
Volume302
Issue number5903
DOIs
StatePublished - Dec 1 1983

ASJC Scopus subject areas

  • General

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