Transcription of DNA injected into Xenopus oocytes is influenced by template topology

R. M. Harland; H. Weintraub; S. L. McKnight

doi:10.1038/302038a0

Transcription of DNA injected into Xenopus oocytes is influenced by template topology

R. M. Harland, H. Weintraub, S. L. McKnight

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Abstract

We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.

Original language	English (US)
Pages (from-to)	38-43
Number of pages	6
Journal	Nature
Volume	302
Issue number	5903
DOIs	https://doi.org/10.1038/302038a0
State	Published - 1983

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title = "Transcription of DNA injected into Xenopus oocytes is influenced by template topology",

abstract = "We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.",

author = "Harland, {R. M.} and H. Weintraub and McKnight, {S. L.}",

year = "1983",

doi = "10.1038/302038a0",

language = "English (US)",

volume = "302",

pages = "38--43",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "5903",

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T1 - Transcription of DNA injected into Xenopus oocytes is influenced by template topology

AU - Harland, R. M.

AU - Weintraub, H.

AU - McKnight, S. L.

PY - 1983

Y1 - 1983

N2 - We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.

AB - We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.

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DO - 10.1038/302038a0

M3 - Article

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AN - SCOPUS:0020660108

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VL - 302

SP - 38

EP - 43

JO - Nature

JF - Nature

IS - 5903

ER -

Transcription of DNA injected into Xenopus oocytes is influenced by template topology

Abstract

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