Transcription of DNA injected into Xenopus oocytes is influenced by template topology

R. M. Harland, H. Weintraub, S. L. McKnight

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.

Original languageEnglish (US)
Pages (from-to)38-43
Number of pages6
JournalNature
Volume302
Issue number5903
DOIs
StatePublished - 1983

Fingerprint

Xenopus
Oocytes
Plasmids
DNA
Thymidine Kinase
Simplexvirus
Injections
Genes

ASJC Scopus subject areas

  • General

Cite this

Transcription of DNA injected into Xenopus oocytes is influenced by template topology. / Harland, R. M.; Weintraub, H.; McKnight, S. L.

In: Nature, Vol. 302, No. 5903, 1983, p. 38-43.

Research output: Contribution to journalArticle

Harland, R. M. ; Weintraub, H. ; McKnight, S. L. / Transcription of DNA injected into Xenopus oocytes is influenced by template topology. In: Nature. 1983 ; Vol. 302, No. 5903. pp. 38-43.
@article{9fa87d0d7ccc448ba6ad6e4761b2b92c,
title = "Transcription of DNA injected into Xenopus oocytes is influenced by template topology",
abstract = "We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.",
author = "Harland, {R. M.} and H. Weintraub and McKnight, {S. L.}",
year = "1983",
doi = "10.1038/302038a0",
language = "English (US)",
volume = "302",
pages = "38--43",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "5903",

}

TY - JOUR

T1 - Transcription of DNA injected into Xenopus oocytes is influenced by template topology

AU - Harland, R. M.

AU - Weintraub, H.

AU - McKnight, S. L.

PY - 1983

Y1 - 1983

N2 - We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.

AB - We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.

UR - http://www.scopus.com/inward/record.url?scp=0020660108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020660108&partnerID=8YFLogxK

U2 - 10.1038/302038a0

DO - 10.1038/302038a0

M3 - Article

C2 - 6828158

AN - SCOPUS:0020660108

VL - 302

SP - 38

EP - 43

JO - Nature

JF - Nature

SN - 0028-0836

IS - 5903

ER -