Transcription termination in vitro at the tryptophan operon attenuator is controlled by secondary structures in the leader transcript.

I. Stroynowski, M. Kuroda, C. Yanofsky

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The role of alternative RNA secondary structures in regulating transcription termination at the attenuator of the tryptophan (trp) operon of Serratia marcescens was examined in vitro by transcribing mutant DNA templates having deletions of different segments of the trp leader region. Deletions that removed sequences corresponding to successive segments of postulated RNA secondary structures either increased or decreased transcription termination at the attenuator. The results obtained are consistent with the hypothesis that transcription termination results from RNA polymerase recognition of a particular RNA secondary structure, the terminator. This structure forms only in the absence of an alternative, preceding, RNA secondary structure, the antiterminator.

Original languageEnglish (US)
Pages (from-to)2206-2210
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number8
Publication statusPublished - Apr 1983

    Fingerprint

ASJC Scopus subject areas

  • General
  • Genetics

Cite this