Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF

E. K. Sullivan, C. S. Weirich, J. R. Guyon, S. Sif, R. E. Kingston

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Chromatin remodeling complexes such as SWI/SNF use the energy of ATP hydrolysis to remodel nucleosomal DNA and increase transcription of nucleosomal templates. Human heat shock factor one (hHSF1) is a tightly regulated activator that stimulates transcriptional initiation and elongation using different portions of its activation domains. Here we demonstrate that hHSF1 associates with BRG1, the ATPase subunit of human SWI/SNF (hSWI/SNF) at endogenous protein concentrations. We also show that hHSF1 activation domains recruit hSWI/SNF to a chromatin template in a purified system. Mutation of hHSF1 residues responsible for activation of transcriptional elongation has the most severe effect on recruitment of SWI/SNF and association of hHSF1 with BRG1, suggesting that recruitment of chromatin remodeling activity might play a role in stimulation of elongation.

Original languageEnglish (US)
Pages (from-to)5826-5837
Number of pages12
JournalMolecular and Cellular Biology
Volume21
Issue number17
DOIs
StatePublished - Aug 22 2001

Fingerprint

Transcriptional Activation
Shock
Chromatin Assembly and Disassembly
Hot Temperature
Chromatin
Adenosine Triphosphatases
Hydrolysis
Adenosine Triphosphate
Mutation
DNA
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF. / Sullivan, E. K.; Weirich, C. S.; Guyon, J. R.; Sif, S.; Kingston, R. E.

In: Molecular and Cellular Biology, Vol. 21, No. 17, 22.08.2001, p. 5826-5837.

Research output: Contribution to journalArticle

Sullivan, E. K. ; Weirich, C. S. ; Guyon, J. R. ; Sif, S. ; Kingston, R. E. / Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF. In: Molecular and Cellular Biology. 2001 ; Vol. 21, No. 17. pp. 5826-5837.
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