TY - JOUR
T1 - Transcriptional analysis and preliminary characterization of ORF Bm42 from Bombyx mori nucleopolyhedrovirus
AU - Acharya, Asha
AU - Gopinathan, Karumathil P.
N1 - Funding Information:
We thank Dr. Veena K Parnaik for anti-lamin antibodies, the Confocal microscope facility at Centre for Cellular and Molecular Biology, Hyderabad, and the automated DNA sequencing facility at our Institute. The technical assistance from Mansdur Rahman and Sangeeta Dha-wan in preparation of the manuscript is gratefully acknowledged. We also thank the Department of Biotechnology, Government of India, and the Council of Scientific and Industrial Research, New Delhi for financial support.
PY - 2002
Y1 - 2002
N2 - Clusters of closely spaced genes are frequently present in baculovirus genomes. We report here the detailed transcriptional mapping of one such gene cluster from Bombyx mori nucleopolyhedrovirus (BmNPV) comprising five ORFs, viz., Bm42, Lef-10, VP1054, Bm44, and Bm45, which are closely spaced and transcribed in the same orientation. This region is homologous to ORFs 53, 53a, 54, 55, and 56, respectively, on the AcMNPV genome. Multiple, overlapping sets of polycistronic transcripts from this region, that initiate independently but terminate at a common 3′ end, were detected following BmNPV infection. Expression of these genes followed a temporal pattern where the shorter transcripts initiating downstream appeared early, and with the progress of infection the longer transcripts initiated from upstream. The first ORF in this cluster, Bm42, is conserved in almost all baculoviruses, but has not been characterized so far. The protein was localized in the cytosol, predominantly near the nucleus-cytoplasm boundary in BmNPV-infected BmN cells. Bm42 was present in the budded virions and was not associated with occluded virus particles or the polyhedral matrix.
AB - Clusters of closely spaced genes are frequently present in baculovirus genomes. We report here the detailed transcriptional mapping of one such gene cluster from Bombyx mori nucleopolyhedrovirus (BmNPV) comprising five ORFs, viz., Bm42, Lef-10, VP1054, Bm44, and Bm45, which are closely spaced and transcribed in the same orientation. This region is homologous to ORFs 53, 53a, 54, 55, and 56, respectively, on the AcMNPV genome. Multiple, overlapping sets of polycistronic transcripts from this region, that initiate independently but terminate at a common 3′ end, were detected following BmNPV infection. Expression of these genes followed a temporal pattern where the shorter transcripts initiating downstream appeared early, and with the progress of infection the longer transcripts initiated from upstream. The first ORF in this cluster, Bm42, is conserved in almost all baculoviruses, but has not been characterized so far. The protein was localized in the cytosol, predominantly near the nucleus-cytoplasm boundary in BmNPV-infected BmN cells. Bm42 was present in the budded virions and was not associated with occluded virus particles or the polyhedral matrix.
KW - Baculovirus
KW - BmnPV
KW - Immunolocalization
KW - Polycistronic message
KW - Promoter occlusion
KW - Transcriptional regulation
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U2 - 10.1006/viro.2002.1525
DO - 10.1006/viro.2002.1525
M3 - Article
C2 - 12202224
AN - SCOPUS:0036434123
SN - 0042-6822
VL - 299
SP - 213
EP - 224
JO - Virology
JF - Virology
IS - 2
ER -