Transcriptional analysis of inhibition of lipopolysaccharide response by anti-IgM

Bethany Beilue Moore, Kiyoshi Ariizumi, Philip W. Tucker, Dorothy Yuan

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Abstract

Murine splenic B cells, when stimulated with LPS, show a generalized enhancement of gene transcription. In addition to this general increase, there is a specific increase in μs mRNA production and differentiation to high rate IgM secretion. Anti-μ added concomitantly with LPS at the start of culture has been demonstrated to inhibit the LPS-induced increase in us mRNA production without affecting the proliferative capacity of the cells. By "run-on" analysis of nascent transcription, we have shown that the effect of anti-μ is mediated by the abrogation of the up-regulation of transcription of the μ-gene induced by LPS. Furthermore, by assessing the site of transcription termination, it is possible to infer that alterations in 3′-end processing induced by LPS are also inhibited. We have also found that CAT3 gene activity driven by a number of promoter/enhancers with diverse regulatory motifs are inhibited by anti-μ. These results suggest that the effect of anti-μ cannot be restricted to interactions with a single regulatory element. Therefore, cross-linking of surface IgM may affect a number of genes involved in differentiation to Ig secretion.

Original languageEnglish (US)
Pages (from-to)3366-3374
Number of pages9
JournalJournal of Immunology
Volume150
Issue number8 PART 1
Publication statusPublished - 1993

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ASJC Scopus subject areas

  • Immunology

Cite this

Moore, B. B., Ariizumi, K., Tucker, P. W., & Yuan, D. (1993). Transcriptional analysis of inhibition of lipopolysaccharide response by anti-IgM. Journal of Immunology, 150(8 PART 1), 3366-3374.