TY - JOUR
T1 - Transcriptional autorepression of the stress-inducible gene ATF3
AU - Wolfgang, Curt D.
AU - Liang, Guosheng
AU - Okamoto, Yoshichika
AU - Allen, Amy E.
AU - Hai, Tsonwin
PY - 2000/6/2
Y1 - 2000/6/2
N2 - Previously, we demonstrated that ATF3 (activating transcription factor- 3) is a stress-inducible gene, and the protein it encodes is a transcriptional repressor. In this report, we present evidence suggesting that ATF3 represses the transcription of its own gene. Interestingly, efficient repression requires a consensus ATF/cAMP-responsive element site in the promoter and a previously unidentified ATF3-binding site immediately downstream from the TATA box. Although this new site resembles the known ATF/cAMP-responsive element sequences at the flanking sequence, it differs from them at the center key residues. These observations indicate that ATF3 can tolerate variations in the center of the binding sites if the flanking sequences are favorable. The repression of the ATF3 promoter by its own gene product provides a mechanistic explanation, at least in part, for the transient expression pattern of the ATF3 gene upon stress induction.
AB - Previously, we demonstrated that ATF3 (activating transcription factor- 3) is a stress-inducible gene, and the protein it encodes is a transcriptional repressor. In this report, we present evidence suggesting that ATF3 represses the transcription of its own gene. Interestingly, efficient repression requires a consensus ATF/cAMP-responsive element site in the promoter and a previously unidentified ATF3-binding site immediately downstream from the TATA box. Although this new site resembles the known ATF/cAMP-responsive element sequences at the flanking sequence, it differs from them at the center key residues. These observations indicate that ATF3 can tolerate variations in the center of the binding sites if the flanking sequences are favorable. The repression of the ATF3 promoter by its own gene product provides a mechanistic explanation, at least in part, for the transient expression pattern of the ATF3 gene upon stress induction.
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U2 - 10.1074/jbc.M909637199
DO - 10.1074/jbc.M909637199
M3 - Article
C2 - 10748147
AN - SCOPUS:0034596055
SN - 0021-9258
VL - 275
SP - 16865
EP - 16870
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 22
ER -