Abstract
Transcriptional control signals of a model eukaryotic protein-coding gene have been identified by a new procedure of in vitro mutagenesis. This method allows small clusters of nucleotide residues to be substituted in a site-directed manner without causing the addition or deletion of other sequences. Transcription assays of a systematic series of these clustered point mutants have led to the identification of three distinct control signals located within the 105-nucleotide residues immediately upstream from the point where transcription begins.
Original language | English (US) |
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Pages (from-to) | 316-324 |
Number of pages | 9 |
Journal | Science |
Volume | 217 |
Issue number | 4557 |
DOIs | |
State | Published - 1982 |
ASJC Scopus subject areas
- General