Transcriptional control signals of a eukaryotic protein-coding gene

Steven L. McKnight, Robert Kingsbury

Research output: Contribution to journalArticle

717 Citations (Scopus)

Abstract

Transcriptional control signals of a model eukaryotic protein-coding gene have been identified by a new procedure of in vitro mutagenesis. This method allows small clusters of nucleotide residues to be substituted in a site-directed manner without causing the addition or deletion of other sequences. Transcription assays of a systematic series of these clustered point mutants have led to the identification of three distinct control signals located within the 105-nucleotide residues immediately upstream from the point where transcription begins.

Original languageEnglish (US)
Pages (from-to)316-324
Number of pages9
JournalScience
Volume217
Issue number4557
StatePublished - 1982

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Nucleotides
Sequence Deletion
Mutagenesis
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Transcriptional control signals of a eukaryotic protein-coding gene. / McKnight, Steven L.; Kingsbury, Robert.

In: Science, Vol. 217, No. 4557, 1982, p. 316-324.

Research output: Contribution to journalArticle

McKnight, SL & Kingsbury, R 1982, 'Transcriptional control signals of a eukaryotic protein-coding gene', Science, vol. 217, no. 4557, pp. 316-324.
McKnight, Steven L. ; Kingsbury, Robert. / Transcriptional control signals of a eukaryotic protein-coding gene. In: Science. 1982 ; Vol. 217, No. 4557. pp. 316-324.
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