Transcriptional regulation by miRNA mimics that target sequences downstream of gene termini

Scott T. Younger, David R. Corey

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Transcriptome studies have revealed that protein-coding loci within the human genome are overlapped at their 3′-termini by noncoding RNA (ncRNA) transcripts. Small duplex RNAs designed to be fully complementary to these 3′ ncRNAs can modulate transcription of the upstream gene. Robust regulation by designed RNAs suggests that endogenous small RNAs might also recognize 3′ ncRNAs and regulate gene expression. A genome-wide evaluation revealed that sequences immediately downstream of protein-coding genes are enriched with miRNA target sites. We experimentally tested miRNA mimics complementary to the well-characterized 3′-terminus of the human progesterone receptor (PR) gene and observed inhibition of PR transcription. These results suggest that recognition of ncRNA transcripts that overlap gene termini may be a natural function of endogenous small RNAs.

Original languageEnglish (US)
Pages (from-to)2383-2388
Number of pages6
JournalMolecular BioSystems
Volume7
Issue number8
DOIs
StatePublished - Aug 1 2011

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MicroRNAs
RNA
Untranslated RNA
Progesterone Receptors
Genes
Human Genome
Transcriptome
Proteins
Genome
Gene Expression

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Transcriptional regulation by miRNA mimics that target sequences downstream of gene termini. / Younger, Scott T.; Corey, David R.

In: Molecular BioSystems, Vol. 7, No. 8, 01.08.2011, p. 2383-2388.

Research output: Contribution to journalArticle

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