Transcriptional regulation by small RNAs at sequences downstream from 3-2 gene termini

Xuan Yue, Jacob C. Schwartz, Yongjun Chu, Scott T. Younger, Keith T. Gagnon, Sayda Elbashir, Bethany A. Janowski, David R. Corey

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Transcriptome studies reveal many noncoding transcripts overlapping 3-2 gene termini. The function of these transcripts is unknown. Here we have characterized transcription at the progesterone receptor (PR) locus and identified noncoding transcripts that overlap the 3-2 end of the gene. Small RNAs complementary to sequences beyond the 3-2 terminus of PR mRNA modulated expression of PR, recruited argonaute 2 to a 3-2 noncoding transcript, altered occupancy of RNA polymerase II, induced chromatin changes at the PR promoter and affected responses to physiological stimuli. We found that the promoter and 3-2 terminal regions of the PR locus are in close proximity, providing a potential mechanism for RNA-mediated control of transcription over long genomic distances. These results extend the potential for small RNAs to regulate transcription to target sequences beyond the 3-2 termini of mRNA.

Original languageEnglish (US)
Pages (from-to)621-629
Number of pages9
JournalNature Chemical Biology
Volume6
Issue number8
DOIs
StatePublished - Aug 2010

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Progesterone Receptors
Genes
RNA
Complementary RNA
Messenger RNA
RNA Polymerase II
Transcriptome
Chromatin

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Transcriptional regulation by small RNAs at sequences downstream from 3-2 gene termini. / Yue, Xuan; Schwartz, Jacob C.; Chu, Yongjun; Younger, Scott T.; Gagnon, Keith T.; Elbashir, Sayda; Janowski, Bethany A.; Corey, David R.

In: Nature Chemical Biology, Vol. 6, No. 8, 08.2010, p. 621-629.

Research output: Contribution to journalArticle

Yue, X, Schwartz, JC, Chu, Y, Younger, ST, Gagnon, KT, Elbashir, S, Janowski, BA & Corey, DR 2010, 'Transcriptional regulation by small RNAs at sequences downstream from 3-2 gene termini', Nature Chemical Biology, vol. 6, no. 8, pp. 621-629. https://doi.org/10.1038/nchembio.400
Yue, Xuan ; Schwartz, Jacob C. ; Chu, Yongjun ; Younger, Scott T. ; Gagnon, Keith T. ; Elbashir, Sayda ; Janowski, Bethany A. ; Corey, David R. / Transcriptional regulation by small RNAs at sequences downstream from 3-2 gene termini. In: Nature Chemical Biology. 2010 ; Vol. 6, No. 8. pp. 621-629.
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