Abstract
RNAi using single-strand RNA would provide new options for therapeutic development and for investigating critical questions of mechanism. Using chemically modified single-strands, we test the hypothesis that single-stranded RNAs can engage the RNAi pathway and silence gene transcription. We find that a chemically modified single-stranded silencing RNA (ss-siRNA) designed to be complementary to a long noncoding RNA (lncRNA) requires argonaute protein, functions through the RNAi pathway, and inhibits gene transcription. These data expand the use of single-stranded RNA to cell nuclei.
Original language | English (US) |
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Pages (from-to) | 122-126 |
Number of pages | 5 |
Journal | ACS chemical biology |
Volume | 8 |
Issue number | 1 |
DOIs | |
State | Published - Jan 18 2013 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine