Transcriptional silencing by single-stranded RNAs targeting a noncoding rna that overlaps a gene promoter

Masayuki Matsui, Thazha P. Prakash, David R. Corey

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

RNAi using single-strand RNA would provide new options for therapeutic development and for investigating critical questions of mechanism. Using chemically modified single-strands, we test the hypothesis that single-stranded RNAs can engage the RNAi pathway and silence gene transcription. We find that a chemically modified single-stranded silencing RNA (ss-siRNA) designed to be complementary to a long noncoding RNA (lncRNA) requires argonaute protein, functions through the RNAi pathway, and inhibits gene transcription. These data expand the use of single-stranded RNA to cell nuclei.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalACS chemical biology
Volume8
Issue number1
DOIs
StatePublished - Jan 18 2013

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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