Transcriptional silencing of the RUNX3 gene by CpG hypermethylation is associated with lung cancer

Qing Lin Li; Hye Ryeon Kim; Wun Jae Kim; Joong Kook Choi; Yong Hee Lee; Hwan Mook Kim; Long Shan Li; Hoguen Kim; Joon Chang; Yoshiaki Ito; Kwang Youl Lee; Suk Chul Bae

doi:10.1016/j.bbrc.2003.12.079

Transcriptional silencing of the RUNX3 gene by CpG hypermethylation is associated with lung cancer

Qing Lin Li, Hye Ryeon Kim, Wun Jae Kim, Joong Kook Choi, Yong Hee Lee, Hwan Mook Kim, Long Shan Li, Hoguen Kim, Joon Chang, Yoshiaki Ito, Kwang Youl Lee, Suk Chul Bae

Hamon Center Therapeutic Oncology - Minna Lab

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

RUNX family transcription factors are integral components of TGF-β signaling pathways and have been implicated in cell cycle regulation, differentiation, apoptosis, and malignant transformation. It was noted previously that allele loss and loss of expression of RUNX3 are causally involved in gastric carcinogenesis. Our results demonstrate that RUNX3 is inactivated by aberrant DNA methylation in approximately 19% of lung cancer cell lines and 24% of primary lung cancer specimens. RUNX3 methylation is tumor-specific, since it is not observed in surrounding normal lung tissues. Our results suggest that loss of RUNX3 expression by DNA hypermethylation is frequently associated with the evolution of lung cancer.

Original language	English (US)
Pages (from-to)	223-228
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	314
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2003.12.079
State	Published - Jan 30 2004

Keywords

Lung cancer
Methylation
RUNX3
TGF-β
Transcription

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2003.12.079

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title = "Transcriptional silencing of the RUNX3 gene by CpG hypermethylation is associated with lung cancer",

abstract = "RUNX family transcription factors are integral components of TGF-β signaling pathways and have been implicated in cell cycle regulation, differentiation, apoptosis, and malignant transformation. It was noted previously that allele loss and loss of expression of RUNX3 are causally involved in gastric carcinogenesis. Our results demonstrate that RUNX3 is inactivated by aberrant DNA methylation in approximately 19% of lung cancer cell lines and 24% of primary lung cancer specimens. RUNX3 methylation is tumor-specific, since it is not observed in surrounding normal lung tissues. Our results suggest that loss of RUNX3 expression by DNA hypermethylation is frequently associated with the evolution of lung cancer.",

keywords = "Lung cancer, Methylation, RUNX3, TGF-β, Transcription",

author = "Li, {Qing Lin} and Kim, {Hye Ryeon} and Kim, {Wun Jae} and Choi, {Joong Kook} and {Hee Lee}, Yong and Kim, {Hwan Mook} and {Shan Li}, Long and Hoguen Kim and Joon Chang and Yoshiaki Ito and {Youl Lee}, Kwang and Bae, {Suk Chul}",

note = "Funding Information: This work was supported by Korea Research Foundation Grant (KRF-2002-042-C00062) and the Creative Research Program (M10301000012) of the Ministry of Science and Technology of Korea to S.C. Bae.",

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doi = "10.1016/j.bbrc.2003.12.079",

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T1 - Transcriptional silencing of the RUNX3 gene by CpG hypermethylation is associated with lung cancer

AU - Li, Qing Lin

AU - Kim, Hye Ryeon

AU - Kim, Wun Jae

AU - Choi, Joong Kook

AU - Hee Lee, Yong

AU - Kim, Hwan Mook

AU - Shan Li, Long

AU - Kim, Hoguen

AU - Chang, Joon

AU - Ito, Yoshiaki

AU - Youl Lee, Kwang

AU - Bae, Suk Chul

N1 - Funding Information: This work was supported by Korea Research Foundation Grant (KRF-2002-042-C00062) and the Creative Research Program (M10301000012) of the Ministry of Science and Technology of Korea to S.C. Bae.

PY - 2004/1/30

Y1 - 2004/1/30

N2 - RUNX family transcription factors are integral components of TGF-β signaling pathways and have been implicated in cell cycle regulation, differentiation, apoptosis, and malignant transformation. It was noted previously that allele loss and loss of expression of RUNX3 are causally involved in gastric carcinogenesis. Our results demonstrate that RUNX3 is inactivated by aberrant DNA methylation in approximately 19% of lung cancer cell lines and 24% of primary lung cancer specimens. RUNX3 methylation is tumor-specific, since it is not observed in surrounding normal lung tissues. Our results suggest that loss of RUNX3 expression by DNA hypermethylation is frequently associated with the evolution of lung cancer.

AB - RUNX family transcription factors are integral components of TGF-β signaling pathways and have been implicated in cell cycle regulation, differentiation, apoptosis, and malignant transformation. It was noted previously that allele loss and loss of expression of RUNX3 are causally involved in gastric carcinogenesis. Our results demonstrate that RUNX3 is inactivated by aberrant DNA methylation in approximately 19% of lung cancer cell lines and 24% of primary lung cancer specimens. RUNX3 methylation is tumor-specific, since it is not observed in surrounding normal lung tissues. Our results suggest that loss of RUNX3 expression by DNA hypermethylation is frequently associated with the evolution of lung cancer.

KW - Lung cancer

KW - Methylation

KW - RUNX3

KW - TGF-β

KW - Transcription

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Transcriptional silencing of the RUNX3 gene by CpG hypermethylation is associated with lung cancer

Abstract

Keywords

ASJC Scopus subject areas

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