@article{fd1c5579fc7447229d93ee699ad0e47f,
title = "Transcriptomic Analysis of Single Isolated Myofibers Identifies miR-27a-3p and miR-142-3p as Regulators of Metabolism in Skeletal Muscle",
abstract = "Skeletal muscle is composed of different myofiber types that preferentially use glucose or lipids for ATP production. How fuel preference is regulated in these post-mitotic cells is largely unknown, making this issue a key question in the fields of muscle and whole-body metabolism. Here, we show that microRNAs (miRNAs) play a role in defining myofiber metabolic profiles. mRNA and miRNA signatures of all myofiber types obtained at the single-cell level unveiled fiber-specific regulatory networks and identified two master miRNAs that coordinately control myofiber fuel preference and mitochondrial morphology. Our work provides a complete and integrated mouse myofiber type-specific catalog of gene and miRNA expression and establishes miR-27a-3p and miR-142-3p as regulators of lipid use in skeletal muscle. Chemello et al. characterize coding mRNAs and non-coding microRNAs expressed by myofibers of hindlimb mouse muscles, identifying complex interactions between these molecules that modulate mitochondrial functions and muscle metabolism. They demonstrate that specific short non-coding RNAs influence the contractile fiber composition of skeletal muscles by modulating muscle metabolism.",
keywords = "lipids, miRNAs, mitochondria, single myofiber, skeletal muscle metabolism",
author = "Francesco Chemello and Francesca Grespi and Alessandra Zulian and Pasqua Cancellara and Etienne Hebert-Chatelain and Paolo Martini and Camilla Bean and Enrico Alessio and Lisa Buson and Martina Bazzega and Andrea Armani and Marco Sandri and Ruggero Ferrazza and Paolo Laveder and Graziano Guella and Carlo Reggiani and Chiara Romualdi and Paolo Bernardi and Luca Scorrano and Stefano Cagnin and Gerolamo Lanfranchi",
note = "Funding Information: We wish to thank Dr. Federico Caicci (BioImaging Facility of the Department of Biology, University of Padova) for help with electron microscopy analyses; Dr. Caterina Millino and Dr. Beniamina Pacchioni (Microarray Service MicroCribi, CRIBI Biotechnology Centre, University of Padova) for the help with microarray analyses; Anna Stocco for her commitment in performing preliminary experiments; Professor Fabio Di Lisa and Dr. Valeria Petronilli for useful discussions; and Dr. Kelly Carroll and Dr. Catherine Makarewich for critical reading of the manuscript. This work was supported by grants from the CARIPARO Foundation ( FIBRE-GEXP ) and the University of Padova ( CPDA139317 ) to S.C. and G.L.; the Cariplo Foundation ( 2016-1006 ) to S.C. and G.L.; the Italian Ministry of Health ( GR-2011-02346845 ) to S.C.; the AIRC Foundation (Italy project IG2015-ID17773 ) to G.L.; an FP7 CIG ( PCIG13-GA-2013-618697 ); the Italian Ministry of Research ( FIRB RBAP11Z3YA_005 ); and a grant from the EFSD/Novo Nordisk Programme for Diabetes Research in Europe to L.S. F.G. and E.H.-C. were supported by a DTI-IMPORT FP7 fellowship. ",
year = "2019",
month = mar,
day = "26",
doi = "10.1016/j.celrep.2019.02.105",
language = "English (US)",
volume = "26",
pages = "3784--3797.e8",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "13",
}