Transepithelial potential difference profile of the distal tubule of the rat kidney

L. J. Barratt, F. C. Rector, J. P. Kokko, C. C. Tisher, D. W. Seldin

Research output: Contribution to journalArticle

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Abstract

In a recent micropuncture study electrodes with relatively large tips (3 to 5 μ O.D.) and hence, low tip resistances were used to measure the transepithelial potential difference (PD) across the proximal tubule of the rat kidney. The present study reexamines the PD of the distal tubule of the rat kidney using such electrodes. In contrast to previous studies where a negative PD has been uniformly found in the tubule, the transtubular PD was found to be previously oriented (+3.7 mv) when particular efforts were made to puncture the earliest accessible segments. In accord with previous observations, the PD of the late segment was consistently negative (mean, -19.6 mv). Morphologic examination of the epithelium at the site of puncture suggests that in the very early distal tubule where positive potentials are recorded, the epithelium is characteristic of the distal convoluted tubule. By contrast, in the latter part of the distal tubule, where negative potentials are recorded, the epithelium displays the morphologic characteristics of the cortical collecting duct. The results of these studies suggest that the net transport properties of the distal tubule, that is the region of the nephron beginning just beyond the macula densa and extending to the first junction with another renal tubule, are a composite of activities of at least two types of epithelium.

Original languageEnglish (US)
Pages (from-to)368-375
Number of pages8
JournalKidney International
Volume8
Issue number6
StatePublished - 1975

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Distal Kidney Tubule
Epithelium
Punctures
Electrodes
Proximal Kidney Tubule
Nephrons
Kidney

ASJC Scopus subject areas

  • Nephrology

Cite this

Barratt, L. J., Rector, F. C., Kokko, J. P., Tisher, C. C., & Seldin, D. W. (1975). Transepithelial potential difference profile of the distal tubule of the rat kidney. Kidney International, 8(6), 368-375.

Transepithelial potential difference profile of the distal tubule of the rat kidney. / Barratt, L. J.; Rector, F. C.; Kokko, J. P.; Tisher, C. C.; Seldin, D. W.

In: Kidney International, Vol. 8, No. 6, 1975, p. 368-375.

Research output: Contribution to journalArticle

Barratt, LJ, Rector, FC, Kokko, JP, Tisher, CC & Seldin, DW 1975, 'Transepithelial potential difference profile of the distal tubule of the rat kidney', Kidney International, vol. 8, no. 6, pp. 368-375.
Barratt LJ, Rector FC, Kokko JP, Tisher CC, Seldin DW. Transepithelial potential difference profile of the distal tubule of the rat kidney. Kidney International. 1975;8(6):368-375.
Barratt, L. J. ; Rector, F. C. ; Kokko, J. P. ; Tisher, C. C. ; Seldin, D. W. / Transepithelial potential difference profile of the distal tubule of the rat kidney. In: Kidney International. 1975 ; Vol. 8, No. 6. pp. 368-375.
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