Transfer plate radioassay using adsorbed anti-insulin antibody to detect insulin secreted by islet cell cultures

Richard M. Scearce, Herbert K. Oie, Adi F. Gazdar, William L. Chick, George S. Eisenbarth

Research output: Contribution to journalArticle

Abstract

A solid-phase radioimmunoassay for detection of insulin synthesized by islet cell clones is described. This assay employs anti-insulin antibody adsorbed onto fenestrated polyvinyl chloride 96-well plates ('transfer plates'). The calibrated aperture in the bottom of each transfer plate well permits fluid to enter the wells when transfer plates are lowered into microculture wells containing insulin. With this assay it is possible to rapidly screen hundreds of islet cell cultures for insulin production. We have used this assay to facilitate cloning of the RIN rat insulinoma cell line. The assay readily detects insulin synthesis by RIN cells and [125I]insulin is not displaced by culture medium from cells which do not produce insulin. The transfer plate format should be applicable to semiautomate other radioimmunoassays.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalJournal of Immunological Methods
Volume42
Issue number1
DOIs
StatePublished - Apr 16 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Transfer plate radioassay using adsorbed anti-insulin antibody to detect insulin secreted by islet cell cultures'. Together they form a unique fingerprint.

  • Cite this