Transforming growth factor-β stimulates the expression of endothelin mRNA by vascular endothelial cells

Hiroki Kurihara; Masao Yoshizumi; Takao Sugiyama; Fumimaro Takaku; Masashi Yanagisawa; Tomoh Masaki; Masaru Hamaoki; Hirohisa Kato; Yoshio Yazaki

doi:10.1016/0006-291X(89)92270-5

Transforming growth factor-β stimulates the expression of endothelin mRNA by vascular endothelial cells

Hiroki Kurihara, Masao Yoshizumi, Takao Sugiyama, Fumimaro Takaku, Masashi Yanagisawa, Tomoh Masaki, Masaru Hamaoki, Hirohisa Kato, Yoshio Yazaki

Research output: Contribution to journal › Article › peer-review

377 Scopus citations

Abstract

Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-β1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endothelial cell conditioned media was also increased by TGF-β1. These results suggest that TGF-β1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.

Original language	English (US)
Pages (from-to)	1435-1440
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	159
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(89)92270-5
State	Published - Mar 31 1989

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Transforming growth factor-β stimulates the expression of endothelin mRNA by vascular endothelial cells. / Kurihara, Hiroki; Yoshizumi, Masao; Sugiyama, Takao; Takaku, Fumimaro; Yanagisawa, Masashi; Masaki, Tomoh; Hamaoki, Masaru; Kato, Hirohisa; Yazaki, Yoshio.

In: Biochemical and Biophysical Research Communications, Vol. 159, No. 3, 31.03.1989, p. 1435-1440.

Research output: Contribution to journal › Article › peer-review

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abstract = "Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-β1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endothelial cell conditioned media was also increased by TGF-β1. These results suggest that TGF-β1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.",

author = "Hiroki Kurihara and Masao Yoshizumi and Takao Sugiyama and Fumimaro Takaku and Masashi Yanagisawa and Tomoh Masaki and Masaru Hamaoki and Hirohisa Kato and Yoshio Yazaki",

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AU - Yanagisawa, Masashi

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AU - Hamaoki, Masaru

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AB - Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-β1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endothelial cell conditioned media was also increased by TGF-β1. These results suggest that TGF-β1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.

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