Transforming growth factor-β stimulates the expression of endothelin mRNA by vascular endothelial cells

Hiroki Kurihara, Masao Yoshizumi, Takao Sugiyama, Fumimaro Takaku, Masashi Yanagisawa, Tomoh Masaki, Masaru Hamaoki, Hirohisa Kato, Yoshio Yazaki

Research output: Contribution to journalArticle

374 Scopus citations

Abstract

Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-β1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endothelial cell conditioned media was also increased by TGF-β1. These results suggest that TGF-β1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.

Original languageEnglish (US)
Pages (from-to)1435-1440
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number3
DOIs
StatePublished - Mar 31 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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