Abstract
Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-β1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endothelial cell conditioned media was also increased by TGF-β1. These results suggest that TGF-β1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1435-1440 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 159 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 31 1989 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Transforming growth factor-β stimulates the expression of endothelin mRNA by vascular endothelial cells. / Kurihara, Hiroki; Yoshizumi, Masao; Sugiyama, Takao; Takaku, Fumimaro; Yanagisawa, Masashi; Masaki, Tomoh; Hamaoki, Masaru; Kato, Hirohisa; Yazaki, Yoshio.
In: Biochemical and Biophysical Research Communications, Vol. 159, No. 3, 31.03.1989, p. 1435-1440.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transforming growth factor-β stimulates the expression of endothelin mRNA by vascular endothelial cells
AU - Kurihara, Hiroki
AU - Yoshizumi, Masao
AU - Sugiyama, Takao
AU - Takaku, Fumimaro
AU - Yanagisawa, Masashi
AU - Masaki, Tomoh
AU - Hamaoki, Masaru
AU - Kato, Hirohisa
AU - Yazaki, Yoshio
PY - 1989/3/31
Y1 - 1989/3/31
N2 - Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-β1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endothelial cell conditioned media was also increased by TGF-β1. These results suggest that TGF-β1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.
AB - Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-β1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endothelial cell conditioned media was also increased by TGF-β1. These results suggest that TGF-β1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.
UR - http://www.scopus.com/inward/record.url?scp=0024521958&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024521958&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)92270-5
DO - 10.1016/0006-291X(89)92270-5
M3 - Article
C2 - 2649101
AN - SCOPUS:0024521958
VL - 159
SP - 1435
EP - 1440
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -