Transgenic wheat progenies resistant to powdery mildew generated by Agrobacterium transformation of barley Mlo anti-sense gene

Tong Jin Zhao, Heng Liu, Shuang Yi Zhao, Hui Min Chen, Guang Min Xia

Research output: Contribution to journalArticle

Abstract

Complete coding region sequence of Mlo cDNA from barley cultivar 'Stirling' was cloned and inserted into the BamHl site of binary vector pBI121.2, driven by 35S promotor in reverse direction. Two wheat (Triticum aestivum L.) genotypes 'Yanyou 2801' and ' Yanyou361' were transformed by Agrobacterium tumefaciens with shoot apical meristem transformation method. A total of 55 T0 transgenic plants were obtained, and the average transformation frequency was as high as 13.58%, Southern blot proved Mlo gene was integrated into T0 wheat genome and inherited to T1, progenies. The T0 and T1 transgenic plants of both genotypes showed higher resistance to powdery mildew than the controls in both green house and field. The shoot apical meristem transformation with A. tumefaciens is a simple, dependable and efficient method to generate transgenic wheat. It can overcome the difficult of the restriction to transformation of different wheat genotypes, and can get rid of the somoclonal variations due to tissue culture of immature embryos and calli.

Original languageEnglish (US)
Pages (from-to)731-736
Number of pages6
JournalPlant Physiology Communications
Volume46
Issue number7
StatePublished - Jul 1 2010

Keywords

  • Agrobacterium transformation
  • Barley Mlo anti-sense gene
  • Powdery mildew
  • Transgenic wheat

ASJC Scopus subject areas

  • Forestry
  • Ecology
  • Molecular Biology
  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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