Transient adhesion of platelets in pump-oxygenator systems: Influence of SMA and nitric oxide treatments

J. Li, M. K. Sly, R. Chao, A. Constantinescu, Padmakar V Kulkarni, F. H. Wians, Michael E Jessen, R. C. Eberhart

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We employed gamma scintigraphy to quantify the transient accumulations of platelets in pump-oxygenator systems employed in cardiopulmonary bypass (CPB). A flat sheet microporous polypropylene membrane oxygenator (Cobe Duo) was employed, with and without siloxane/caprolactone oligomer coating (SMA) (n = 8 each). The effect of nitric oxide gas infusion on platelet deposition was also evaluated for the uncoated Cobe Duo system (n = 10 each). Scintigraphic images of radiolabelled cells were obtained and converted to numbers of all platelets, labeled and unlabeled, adhering to the pump and oxygenator surfaces. These numbers were compared, by study group, for a 90-min period of normothermic CPB in the adult pig, employing standard prime and anticoagulation regimens. Platelets adhered in large numbers to control oxygenators, reaching maxima (> 20% of the circulating platelet mass) 30 min following institution of CPB, and decreasing for the duration of CPB. SMA treatment significantly decreased platelet adhesion following a 5-l0-min transient accumulation period. Nitric oxide infusion significantly reduced platelet adhesion throughout the CPB period. Platelet accumulations on the high fluid shear centrifugal pump surfaces increased monotonically to maxima at about the same time as for the oxygenators, but did not decrease thereafter. Higher platelet surface densities were observed on the centrifugal pump surfaces than on the oxygenator surfaces. CPB with the untreated circuit tended to reduce circulating platelet counts vs theoretical values based on hemodilution alone. In contrast, SMA significantly increased the circulating platelet count versus the untreated control group. These results indicate that platelet adherence to the foreign surfaces of CPB equipment are influenced in characteristic ways by time and fluid shear. SMA treatment and nitric oxide infusion both reduce platelet adhesion to oxygenator surfaces. SMA treatment spares these cells for the circulation.

Original languageEnglish (US)
Pages (from-to)235-246
Number of pages12
JournalJournal of Biomaterials Science, Polymer Edition
Volume10
Issue number2
StatePublished - 1999

Fingerprint

Oxygenators
Nitric oxide
Platelets
Nitric Oxide
Blood Platelets
Adhesion
Pumps
Cardiopulmonary Bypass
Platelet Count
Therapeutics
Centrifugal pumps
Membrane Oxygenators
Siloxanes
Hemodilution
Polypropylenes
Radionuclide Imaging
Fluids
Swine
Gases
Oligomers

Keywords

  • Cardiopulmonary bypass
  • Gamma scintigraphy
  • Nitric oxide
  • Platelet adhesion
  • SMA

ASJC Scopus subject areas

  • Biophysics

Cite this

Li, J., Sly, M. K., Chao, R., Constantinescu, A., Kulkarni, P. V., Wians, F. H., ... Eberhart, R. C. (1999). Transient adhesion of platelets in pump-oxygenator systems: Influence of SMA and nitric oxide treatments. Journal of Biomaterials Science, Polymer Edition, 10(2), 235-246.

Transient adhesion of platelets in pump-oxygenator systems : Influence of SMA and nitric oxide treatments. / Li, J.; Sly, M. K.; Chao, R.; Constantinescu, A.; Kulkarni, Padmakar V; Wians, F. H.; Jessen, Michael E; Eberhart, R. C.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 10, No. 2, 1999, p. 235-246.

Research output: Contribution to journalArticle

Li, J, Sly, MK, Chao, R, Constantinescu, A, Kulkarni, PV, Wians, FH, Jessen, ME & Eberhart, RC 1999, 'Transient adhesion of platelets in pump-oxygenator systems: Influence of SMA and nitric oxide treatments', Journal of Biomaterials Science, Polymer Edition, vol. 10, no. 2, pp. 235-246.
Li, J. ; Sly, M. K. ; Chao, R. ; Constantinescu, A. ; Kulkarni, Padmakar V ; Wians, F. H. ; Jessen, Michael E ; Eberhart, R. C. / Transient adhesion of platelets in pump-oxygenator systems : Influence of SMA and nitric oxide treatments. In: Journal of Biomaterials Science, Polymer Edition. 1999 ; Vol. 10, No. 2. pp. 235-246.
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abstract = "We employed gamma scintigraphy to quantify the transient accumulations of platelets in pump-oxygenator systems employed in cardiopulmonary bypass (CPB). A flat sheet microporous polypropylene membrane oxygenator (Cobe Duo) was employed, with and without siloxane/caprolactone oligomer coating (SMA) (n = 8 each). The effect of nitric oxide gas infusion on platelet deposition was also evaluated for the uncoated Cobe Duo system (n = 10 each). Scintigraphic images of radiolabelled cells were obtained and converted to numbers of all platelets, labeled and unlabeled, adhering to the pump and oxygenator surfaces. These numbers were compared, by study group, for a 90-min period of normothermic CPB in the adult pig, employing standard prime and anticoagulation regimens. Platelets adhered in large numbers to control oxygenators, reaching maxima (> 20{\%} of the circulating platelet mass) 30 min following institution of CPB, and decreasing for the duration of CPB. SMA treatment significantly decreased platelet adhesion following a 5-l0-min transient accumulation period. Nitric oxide infusion significantly reduced platelet adhesion throughout the CPB period. Platelet accumulations on the high fluid shear centrifugal pump surfaces increased monotonically to maxima at about the same time as for the oxygenators, but did not decrease thereafter. Higher platelet surface densities were observed on the centrifugal pump surfaces than on the oxygenator surfaces. CPB with the untreated circuit tended to reduce circulating platelet counts vs theoretical values based on hemodilution alone. In contrast, SMA significantly increased the circulating platelet count versus the untreated control group. These results indicate that platelet adherence to the foreign surfaces of CPB equipment are influenced in characteristic ways by time and fluid shear. SMA treatment and nitric oxide infusion both reduce platelet adhesion to oxygenator surfaces. SMA treatment spares these cells for the circulation.",
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