TY - JOUR
T1 - Transient expression of a winged-helix protein, MNF-β, during myogenesis
AU - Yang, Quan
AU - Bassel-Duby, Rhonda
AU - Williams, R. Sanders
PY - 1997/9
Y1 - 1997/9
N2 - A novel winged-helix transcription factor, MNF-β, is expressed coincidentally with cell cycle withdrawal and differentiation of skeletal myogenic cells. MNF-β is closely related to the myocyte nuclear factor (MNF) protein previously described (now termed MNF-α), but expression of the two isoforms is differentially regulated, and they exhibit distinctive functional properties with respect to DNA binding in vitro and transcriptional regulatory activity in transient-transfection assays. A DNA sequence motif binding MNF-β with high affinity was selected from a library of random oligonucleotides and was found to be similar to but distinct from the cognate binding site for HNF-3β, a more distantly related winged-helix protein. The temporal pattern of MNF-β expression and the presence of MNF binding motifs within conserved promoter elements of several genes that modulate cell cycle progression support a working hypothesis that MNF proteins may modulate proliferation of myogenic precursor cells during development and muscle regeneration.
AB - A novel winged-helix transcription factor, MNF-β, is expressed coincidentally with cell cycle withdrawal and differentiation of skeletal myogenic cells. MNF-β is closely related to the myocyte nuclear factor (MNF) protein previously described (now termed MNF-α), but expression of the two isoforms is differentially regulated, and they exhibit distinctive functional properties with respect to DNA binding in vitro and transcriptional regulatory activity in transient-transfection assays. A DNA sequence motif binding MNF-β with high affinity was selected from a library of random oligonucleotides and was found to be similar to but distinct from the cognate binding site for HNF-3β, a more distantly related winged-helix protein. The temporal pattern of MNF-β expression and the presence of MNF binding motifs within conserved promoter elements of several genes that modulate cell cycle progression support a working hypothesis that MNF proteins may modulate proliferation of myogenic precursor cells during development and muscle regeneration.
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U2 - 10.1128/mcb.17.9.5236
DO - 10.1128/mcb.17.9.5236
M3 - Article
C2 - 9271401
AN - SCOPUS:0030741357
SN - 0270-7306
VL - 17
SP - 5236
EP - 5243
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 9
ER -