Transient gene and microRNA expression profile changes of confluent human fibroblast cells in spaceflight

Ye Zhang, Tao Lu, Michael Wong, Xiaoyu Wang, Louis Stodieck, Fathi Karouia, Michael Story, Honglu Wu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Microgravity, or an altered gravity environment different from the 1 g of the Earth, has been shown to influence global gene expression patterns and protein levels in cultured cells. However, most of the reported studies that have been conducted in space or by using simulated microgravity on the ground have focused on the growth or differentiation of these cells. It has not been specifically addressed whether nonproliferating cultured cells will sense the presence of microgravity in space. In an experiment conducted onboard the International Space Station, confluent human fibroblast cells were fixed after being cultured in space for 3 and 14 d, respectively, to investigate changes in gene and microRNA (miRNA) expression profiles in these cells. Results of the experiment showed that on d 3, both the flown and ground cells were still proliferating slowly, as measured by the percentage of Ki-67+ cells. Gene and miRNA expression data indicated activation of NF-κB and other growth-related pathways that involve hepatocyte growth factor and VEGF as well as the down-regulation of the Let-7 miRNA family. On d 14, when the cells were mostly nonproliferating, the gene and miRNA expression profile of the flight sample was indistinguishable from that of the ground sample. Comparison of gene and miRNA expressions in the d 3 samples, with respect to d 14, revealed that most of the changes observed on d 3 were related to cell growth for both the flown and ground cells. Analysis of cytoskeletal changes via immunohistochemistry staining of the cells with antibodies for α-tubulin and fibronectin showed no difference between the flown and ground samples. Taken together, our study suggests that in true nondividing human fibroblast cells in culture, microgravity experienced in space has little effect on gene and miRNA expression profiles.

Original languageEnglish (US)
Pages (from-to)2211-2224
Number of pages14
JournalFASEB Journal
Volume30
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Space Flight
Fibroblasts
MicroRNAs
Transcriptome
Genes
Cells
Microgravity
Weightlessness
Gene Expression
Cultured Cells
Altered Gravity
Hepatocyte Growth Factor
Growth
Cell growth
Space stations
Tubulin
Fibronectins
Cell culture
Gene expression
Vascular Endothelial Growth Factor A

Keywords

  • Cell growth condition
  • Microgravity
  • Omics

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Zhang, Y., Lu, T., Wong, M., Wang, X., Stodieck, L., Karouia, F., ... Wu, H. (2016). Transient gene and microRNA expression profile changes of confluent human fibroblast cells in spaceflight. FASEB Journal, 30(6), 2211-2224. https://doi.org/10.1096/fj.201500121

Transient gene and microRNA expression profile changes of confluent human fibroblast cells in spaceflight. / Zhang, Ye; Lu, Tao; Wong, Michael; Wang, Xiaoyu; Stodieck, Louis; Karouia, Fathi; Story, Michael; Wu, Honglu.

In: FASEB Journal, Vol. 30, No. 6, 01.06.2016, p. 2211-2224.

Research output: Contribution to journalArticle

Zhang, Y, Lu, T, Wong, M, Wang, X, Stodieck, L, Karouia, F, Story, M & Wu, H 2016, 'Transient gene and microRNA expression profile changes of confluent human fibroblast cells in spaceflight', FASEB Journal, vol. 30, no. 6, pp. 2211-2224. https://doi.org/10.1096/fj.201500121
Zhang, Ye ; Lu, Tao ; Wong, Michael ; Wang, Xiaoyu ; Stodieck, Louis ; Karouia, Fathi ; Story, Michael ; Wu, Honglu. / Transient gene and microRNA expression profile changes of confluent human fibroblast cells in spaceflight. In: FASEB Journal. 2016 ; Vol. 30, No. 6. pp. 2211-2224.
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