Transmembrane pH gradients in vivo: Measurements using fluorinated vitamin B6 derivatives

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

It is well recognized that pH plays a significant regulatory role in most cellular processes. Increasingly, there is interest in transmembrane pH gradients, particularly with respect to tumor growth and response to therapy. NMR offers a non-invasive approach to monitoring cellular pH and detecting changes in response to interventions. This review will consider the strengths of various approaches to measuring pH with particular focus on the reporter molecules designed to interrogate the cellular milieu. In particular, fluorinated vitamin B6 derivatives (6-fluoropyridoxol and 6- fluoropyridoxamine) will be described, which for the first time provide a practical non-destructive method to measure simultaneously intra- and extracellular pH, i.e., the transmembrane pH gradient in animals in vivo based on a single reporter molecule.

Original languageEnglish (US)
Pages (from-to)481-499
Number of pages19
JournalCurrent Medicinal Chemistry
Volume6
Issue number6
Publication statusPublished - 1999

    Fingerprint

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this