Transmission distortion and mosaicism in an unusual transgenic mouse pedigree

Richard D. Palmiter, Thomas M. Wilkie, Howard Y. Chen, Ralph L. Brinster

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

We have analyzed the structure, expression, and inheritance of a foreign DNA insert of an unusual transgenic mouse, designated MyK-103. The insert is composed of two copies of pMK (a metallothionein-thymidine kinase fusion gene) oriented as inverted repeats. No methylation of the foreign DNA could be detected. The original transgenic mouse was mosaic; 10% to 20% of both somatic and germ cells contained the plasmid insert. The pMK insert has been stably transmitted by females through five generations; however, expression of thymidine kinase is extremely variable in different members of the pedigree. Although males carrying the pMK insert are fertile, they never transmit the pMK insert. We propose that the foreign DNA has disrupted a gene that must be expressed during haploid stages of spermatogenesis.

Original languageEnglish (US)
Pages (from-to)869-877
Number of pages9
JournalCell
Volume36
Issue number4
DOIs
StatePublished - 1984

Fingerprint

Mosaicism
Thymidine Kinase
Pedigree
Transgenic Mice
Metallothionein
Gene Fusion
Haploidy
DNA
DNA Methylation
Spermatogenesis
Genes
Germ Cells
Plasmids
Methylation
Fusion reactions
Cells

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Transmission distortion and mosaicism in an unusual transgenic mouse pedigree. / Palmiter, Richard D.; Wilkie, Thomas M.; Chen, Howard Y.; Brinster, Ralph L.

In: Cell, Vol. 36, No. 4, 1984, p. 869-877.

Research output: Contribution to journalArticle

Palmiter, Richard D. ; Wilkie, Thomas M. ; Chen, Howard Y. ; Brinster, Ralph L. / Transmission distortion and mosaicism in an unusual transgenic mouse pedigree. In: Cell. 1984 ; Vol. 36, No. 4. pp. 869-877.
@article{db3d74bc636646038a068152efef0342,
title = "Transmission distortion and mosaicism in an unusual transgenic mouse pedigree",
abstract = "We have analyzed the structure, expression, and inheritance of a foreign DNA insert of an unusual transgenic mouse, designated MyK-103. The insert is composed of two copies of pMK (a metallothionein-thymidine kinase fusion gene) oriented as inverted repeats. No methylation of the foreign DNA could be detected. The original transgenic mouse was mosaic; 10{\%} to 20{\%} of both somatic and germ cells contained the plasmid insert. The pMK insert has been stably transmitted by females through five generations; however, expression of thymidine kinase is extremely variable in different members of the pedigree. Although males carrying the pMK insert are fertile, they never transmit the pMK insert. We propose that the foreign DNA has disrupted a gene that must be expressed during haploid stages of spermatogenesis.",
author = "Palmiter, {Richard D.} and Wilkie, {Thomas M.} and Chen, {Howard Y.} and Brinster, {Ralph L.}",
year = "1984",
doi = "10.1016/0092-8674(84)90036-9",
language = "English (US)",
volume = "36",
pages = "869--877",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Transmission distortion and mosaicism in an unusual transgenic mouse pedigree

AU - Palmiter, Richard D.

AU - Wilkie, Thomas M.

AU - Chen, Howard Y.

AU - Brinster, Ralph L.

PY - 1984

Y1 - 1984

N2 - We have analyzed the structure, expression, and inheritance of a foreign DNA insert of an unusual transgenic mouse, designated MyK-103. The insert is composed of two copies of pMK (a metallothionein-thymidine kinase fusion gene) oriented as inverted repeats. No methylation of the foreign DNA could be detected. The original transgenic mouse was mosaic; 10% to 20% of both somatic and germ cells contained the plasmid insert. The pMK insert has been stably transmitted by females through five generations; however, expression of thymidine kinase is extremely variable in different members of the pedigree. Although males carrying the pMK insert are fertile, they never transmit the pMK insert. We propose that the foreign DNA has disrupted a gene that must be expressed during haploid stages of spermatogenesis.

AB - We have analyzed the structure, expression, and inheritance of a foreign DNA insert of an unusual transgenic mouse, designated MyK-103. The insert is composed of two copies of pMK (a metallothionein-thymidine kinase fusion gene) oriented as inverted repeats. No methylation of the foreign DNA could be detected. The original transgenic mouse was mosaic; 10% to 20% of both somatic and germ cells contained the plasmid insert. The pMK insert has been stably transmitted by females through five generations; however, expression of thymidine kinase is extremely variable in different members of the pedigree. Although males carrying the pMK insert are fertile, they never transmit the pMK insert. We propose that the foreign DNA has disrupted a gene that must be expressed during haploid stages of spermatogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0021153792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021153792&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(84)90036-9

DO - 10.1016/0092-8674(84)90036-9

M3 - Article

C2 - 6323025

AN - SCOPUS:0021153792

VL - 36

SP - 869

EP - 877

JO - Cell

JF - Cell

SN - 0092-8674

IS - 4

ER -