Transplantation of cultured human amniotic epithelial cells onto the ocular surface

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Abstract

Purpose. To investigate the possibility of using cultured human amniotic epithelial (HuA) cells for re-establishment of damaged ocular surfaces, since HuA cells do not express HLA-A, B C, DR antigens and showed no rejection signs in multiple clinical trials. Moreover, these cells are similar to other epithelial cells in formation of the attachment complex to the underlying stroma. Methods. HuA cells were obtained by trypsinization of dissected amniotic membranes and cultured in RPMI-1640. Suspensions (5 × 105/ml) of HuA cells were seeded onto the concave surfaces of collagen corneal shields and incubated at 37°C for 3 to 4 days. These HuA cell-laden shields were then placed on either denuded corneal stromal surfaces in organ culture or de-epithelialized corneas from New Zealand albino rabbits. Specimens were collected 24, 48, 72 and 96 hours later and evaluated histologically. RggjjJUs. The transplanted HuA cells were tightly adhered to the recipient corneal stroma after 24 hours incubation. Formation of cell-stroma hemidesmosomes was confirmed by electron microscopy after 24 hours as well. Conclusion. Cultured HuA cells can be successfully transplanted onto the denuded corneal surface and form a stratified sauamous epithelium which adheres tiahtly to underlying stroma by hemidesmosomes.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - 1997

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Transplantation
Epithelial Cells
Hemidesmosomes
Corneal Stroma
Amnion
HLA-A Antigens
HLA-B Antigens
Organ Culture Techniques
Cornea
Suspensions
Electron Microscopy
Collagen
Epithelium
Clinical Trials
Rabbits
Antigens

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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title = "Transplantation of cultured human amniotic epithelial cells onto the ocular surface",
abstract = "Purpose. To investigate the possibility of using cultured human amniotic epithelial (HuA) cells for re-establishment of damaged ocular surfaces, since HuA cells do not express HLA-A, B C, DR antigens and showed no rejection signs in multiple clinical trials. Moreover, these cells are similar to other epithelial cells in formation of the attachment complex to the underlying stroma. Methods. HuA cells were obtained by trypsinization of dissected amniotic membranes and cultured in RPMI-1640. Suspensions (5 × 105/ml) of HuA cells were seeded onto the concave surfaces of collagen corneal shields and incubated at 37°C for 3 to 4 days. These HuA cell-laden shields were then placed on either denuded corneal stromal surfaces in organ culture or de-epithelialized corneas from New Zealand albino rabbits. Specimens were collected 24, 48, 72 and 96 hours later and evaluated histologically. RggjjJUs. The transplanted HuA cells were tightly adhered to the recipient corneal stroma after 24 hours incubation. Formation of cell-stroma hemidesmosomes was confirmed by electron microscopy after 24 hours as well. Conclusion. Cultured HuA cells can be successfully transplanted onto the denuded corneal surface and form a stratified sauamous epithelium which adheres tiahtly to underlying stroma by hemidesmosomes.",
author = "Yu-Guang He and H. Alizadeh and McCulley, {James P} and Cavanagh, {Harrison D} and J. Huang and Niederkorn, {Jerry Y}",
year = "1997",
language = "English (US)",
volume = "38",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
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TY - JOUR

T1 - Transplantation of cultured human amniotic epithelial cells onto the ocular surface

AU - He, Yu-Guang

AU - Alizadeh, H.

AU - McCulley, James P

AU - Cavanagh, Harrison D

AU - Huang, J.

AU - Niederkorn, Jerry Y

PY - 1997

Y1 - 1997

N2 - Purpose. To investigate the possibility of using cultured human amniotic epithelial (HuA) cells for re-establishment of damaged ocular surfaces, since HuA cells do not express HLA-A, B C, DR antigens and showed no rejection signs in multiple clinical trials. Moreover, these cells are similar to other epithelial cells in formation of the attachment complex to the underlying stroma. Methods. HuA cells were obtained by trypsinization of dissected amniotic membranes and cultured in RPMI-1640. Suspensions (5 × 105/ml) of HuA cells were seeded onto the concave surfaces of collagen corneal shields and incubated at 37°C for 3 to 4 days. These HuA cell-laden shields were then placed on either denuded corneal stromal surfaces in organ culture or de-epithelialized corneas from New Zealand albino rabbits. Specimens were collected 24, 48, 72 and 96 hours later and evaluated histologically. RggjjJUs. The transplanted HuA cells were tightly adhered to the recipient corneal stroma after 24 hours incubation. Formation of cell-stroma hemidesmosomes was confirmed by electron microscopy after 24 hours as well. Conclusion. Cultured HuA cells can be successfully transplanted onto the denuded corneal surface and form a stratified sauamous epithelium which adheres tiahtly to underlying stroma by hemidesmosomes.

AB - Purpose. To investigate the possibility of using cultured human amniotic epithelial (HuA) cells for re-establishment of damaged ocular surfaces, since HuA cells do not express HLA-A, B C, DR antigens and showed no rejection signs in multiple clinical trials. Moreover, these cells are similar to other epithelial cells in formation of the attachment complex to the underlying stroma. Methods. HuA cells were obtained by trypsinization of dissected amniotic membranes and cultured in RPMI-1640. Suspensions (5 × 105/ml) of HuA cells were seeded onto the concave surfaces of collagen corneal shields and incubated at 37°C for 3 to 4 days. These HuA cell-laden shields were then placed on either denuded corneal stromal surfaces in organ culture or de-epithelialized corneas from New Zealand albino rabbits. Specimens were collected 24, 48, 72 and 96 hours later and evaluated histologically. RggjjJUs. The transplanted HuA cells were tightly adhered to the recipient corneal stroma after 24 hours incubation. Formation of cell-stroma hemidesmosomes was confirmed by electron microscopy after 24 hours as well. Conclusion. Cultured HuA cells can be successfully transplanted onto the denuded corneal surface and form a stratified sauamous epithelium which adheres tiahtly to underlying stroma by hemidesmosomes.

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