Transplantation of tissue-cultured corneal endothelium

M. M. Jumblatt, D. M. Maurice, James P McCulley

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Cultured endothelial cells have been shown to regain their physiological function when replaced in the rabbit eye. Corneas were wiped free of native endothelium and seeded with cultured cells. After an incubation period, full-thickness buttons were cut from these corneas and transplanted into recipient animals. Clear grafts were obtained only when the donor cells were derived from cultures less than a month old. Light and scanning electron microscopy showed the endothelial cells of these grafts to be present as a slightly irregular monolayer on the posterior surface of the cornea. In corneas made edematous by benzalkonium chloride, the clear graft remained surrounded by thick and cloudy host tissue. In those grafts with 3H-thymidine-labelled cells, radioactivity was limited to the host tissue.

Original languageEnglish (US)
Pages (from-to)1135-1141
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume17
Issue number12
StatePublished - 1978

Fingerprint

Corneal Endothelium
Tissue Transplantation
Cornea
Transplants
Cultured Cells
Endothelial Cells
Benzalkonium Compounds
Electron Scanning Microscopy
Thymidine
Radioactivity
Endothelium
Rabbits
Light

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Transplantation of tissue-cultured corneal endothelium. / Jumblatt, M. M.; Maurice, D. M.; McCulley, James P.

In: Investigative Ophthalmology and Visual Science, Vol. 17, No. 12, 1978, p. 1135-1141.

Research output: Contribution to journalArticle

Jumblatt, M. M. ; Maurice, D. M. ; McCulley, James P. / Transplantation of tissue-cultured corneal endothelium. In: Investigative Ophthalmology and Visual Science. 1978 ; Vol. 17, No. 12. pp. 1135-1141.
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