Transplants of mouse trisomy 16 hippocampus provide a model of Alzheimer's disease neuropathology

Sarah Jane Richards, Jonathan J. Waters, Konrad Beyreuther, Colin L. Masters, Claude M. Wischik, Dennis R. Sparkman, Charles L. White, Carmela R. Abraham, Stephen B. Dunnett

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Alzheimer's disease, which is characterized by amyloid plaques and neurofibrillary tangles, may be attributed to the abnormal expression of gene(s) located on human chromosome 21. Genetic linkage studies have narrowed the region of candidate genes to 21q11.2-21q22 of the long arm of this chromosome. Several single copy sequences within this region, including the amyloid precursor protein (APP), have been mapped to mouse chromosome 16. Reliable strategies exist for breeding Trisomy 16 mice. However, the consequences of developmental overexpression of genes on chromosome 16 have not been previously investigated, because of the lethal effects of this aneuploidy during gestation. In the present report, we employ neural transplantation to study longterm survival and pathogenesis in Trisomy 16 central nervous system tissues. Immunocytochemical staining with antiserum raised against the synthetic APP, β-A4 and α1-antichymotrypsin revealed numerous densely stained cells within hippocampal grafts of Trisomy 16 mice. Similarly, a population of grafted cells were positively stained following incubation with an antiserum raised against components of the pathological neurofibrillary tangle and with the monoclonal antibodies Tau 6.423 and ubiquitin.

Original languageEnglish (US)
Pages (from-to)297-303
Number of pages7
JournalEMBO Journal
Volume10
Issue number2
StatePublished - Feb 20 1991

Keywords

  • Alzheimer's disease
  • Down's syndrome
  • Trisomy 16
  • amyloid
  • neural transplantation
  • neurofibrillary tangles
  • paired helical filaments
  • plaque

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Transplants of mouse trisomy 16 hippocampus provide a model of Alzheimer's disease neuropathology'. Together they form a unique fingerprint.

  • Cite this

    Richards, S. J., Waters, J. J., Beyreuther, K., Masters, C. L., Wischik, C. M., Sparkman, D. R., White, C. L., Abraham, C. R., & Dunnett, S. B. (1991). Transplants of mouse trisomy 16 hippocampus provide a model of Alzheimer's disease neuropathology. EMBO Journal, 10(2), 297-303.