Transplants of mouse trisomy 16 hippocampus provide a model of Alzheimer's disease neuropathology

Sarah Jane Richards, Jonathan J. Waters, Konrad Beyreuther, Colin L. Masters, Claude M. Wischik, Dennis R. Sparkman, Charles L. White, Carmela R. Abraham, Stephen B. Dunnett

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Alzheimer's disease, which is characterized by amyloid plaques and neurofibrillary tangles, may be attributed to the abnormal expression of gene(s) located on human chromosome 21. Genetic linkage studies have narrowed the region of candidate genes to 21q11.2-21q22 of the long arm of this chromosome. Several single copy sequences within this region, including the amyloid precursor protein (APP), have been mapped to mouse chromosome 16. Reliable strategies exist for breeding Trisomy 16 mice. However, the consequences of developmental overexpression of genes on chromosome 16 have not been previously investigated, because of the lethal effects of this aneuploidy during gestation. In the present report, we employ neural transplantation to study long-term survival and pathogenesis in Trisomy 16 central nervous system tissues. Immunocytochemical staining with antiserum raised against the synthetic APP, β-A4 and α1-antichymotrypsin revealed numerous densely stained cells within hippocampal grafts of Trisomy 16 mice. Similarly, a population of grafted cells were positively stained following incubation with an antiserum raised against components of the pathological neurofibrillary tangle and with the monoclonal antibodies Tau 6.423 and ubiquitin.

Original languageEnglish (US)
Pages (from-to)297-303
Number of pages7
JournalEMBO Journal
Volume10
Issue number2
StatePublished - 1991

Fingerprint

Transplants
Chromosomes
Chromosomes, Human, Pair 16
Hippocampus
Alzheimer Disease
Neurofibrillary Tangles
Amyloid beta-Protein Precursor
Genes
Immune Sera
Developmental Genes
Nerve Tissue
Chromosomes, Human, Pair 21
Genetic Linkage
Amyloid Plaques
Human Chromosomes
Aneuploidy
Ubiquitin
Breeding
Neurology
Central Nervous System

Keywords

  • Alzheimer's disease
  • Amyloid
  • Down's syndrome
  • Neural transplantation
  • Neurofibrillary tangles
  • Paired helical filaments
  • Plaque
  • Trisomy 16

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Richards, S. J., Waters, J. J., Beyreuther, K., Masters, C. L., Wischik, C. M., Sparkman, D. R., ... Dunnett, S. B. (1991). Transplants of mouse trisomy 16 hippocampus provide a model of Alzheimer's disease neuropathology. EMBO Journal, 10(2), 297-303.

Transplants of mouse trisomy 16 hippocampus provide a model of Alzheimer's disease neuropathology. / Richards, Sarah Jane; Waters, Jonathan J.; Beyreuther, Konrad; Masters, Colin L.; Wischik, Claude M.; Sparkman, Dennis R.; White, Charles L.; Abraham, Carmela R.; Dunnett, Stephen B.

In: EMBO Journal, Vol. 10, No. 2, 1991, p. 297-303.

Research output: Contribution to journalArticle

Richards, SJ, Waters, JJ, Beyreuther, K, Masters, CL, Wischik, CM, Sparkman, DR, White, CL, Abraham, CR & Dunnett, SB 1991, 'Transplants of mouse trisomy 16 hippocampus provide a model of Alzheimer's disease neuropathology', EMBO Journal, vol. 10, no. 2, pp. 297-303.
Richards SJ, Waters JJ, Beyreuther K, Masters CL, Wischik CM, Sparkman DR et al. Transplants of mouse trisomy 16 hippocampus provide a model of Alzheimer's disease neuropathology. EMBO Journal. 1991;10(2):297-303.
Richards, Sarah Jane ; Waters, Jonathan J. ; Beyreuther, Konrad ; Masters, Colin L. ; Wischik, Claude M. ; Sparkman, Dennis R. ; White, Charles L. ; Abraham, Carmela R. ; Dunnett, Stephen B. / Transplants of mouse trisomy 16 hippocampus provide a model of Alzheimer's disease neuropathology. In: EMBO Journal. 1991 ; Vol. 10, No. 2. pp. 297-303.
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