Transport of hypoxanthine by human diploid skin fibroblasts deficient in hypoxanthine-guanine phosphoribosyltransferase

Ellen Murphy, Mary Jean Carey Holland, Rody P. Cox

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Abstract

Transport of purine bases and nucleosides by a variety of mammalian cell lines is generally accomplished by facilitated diffusion, a non-concentrative, saturable process. However, previous investigators have been unable to detect a saturable component for the transport of hypoxanthine by human fibroblasts deficient in hypoxanthine-guanine phosphoribosyltransferase, implying that in normal cells the enzyme actively participates in transport. In the present study we have used the phenomenon of countertransport to demonstrate the existence of a saturable transport mechanism in hypoxanthine-guanine phosphoribosyltransferase-deficient human diploid skin fibroblasts.

Original languageEnglish (US)
Pages (from-to)461-464
Number of pages4
JournalExperimental Cell Research
Volume108
Issue number2
DOIs
StatePublished - Sep 1977

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ASJC Scopus subject areas

  • Cell Biology

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