TY - JOUR
T1 - Transport of hypoxanthine by human diploid skin fibroblasts deficient in hypoxanthine-guanine phosphoribosyltransferase
AU - Murphy, Ellen
AU - Holland, Mary Jean Carey
AU - Cox, Rody P.
N1 - Funding Information:
The authors are grateful to Dr M. A. Rich for his support and encouragement during the course of these studies and Dr. S. B. Horowitz for his critical comments concerning the manuscript. This work was supported by grant CA-14100 from the NCI, DHEW, and an institutional grant to the Michigan Cancer Foundation from the United Foundation of Greater Detroit.
Funding Information:
This work was supported by a research grant from the NIH (AM 14528). E.M. is a Genetics Trainee (Training Grant GM 00047); M. J. C. H. is an Arthritis Foundation Fellow.
PY - 1977/9
Y1 - 1977/9
N2 - Transport of purine bases and nucleosides by a variety of mammalian cell lines is generally accomplished by facilitated diffusion, a non-concentrative, saturable process. However, previous investigators have been unable to detect a saturable component for the transport of hypoxanthine by human fibroblasts deficient in hypoxanthine-guanine phosphoribosyltransferase, implying that in normal cells the enzyme actively participates in transport. In the present study we have used the phenomenon of countertransport to demonstrate the existence of a saturable transport mechanism in hypoxanthine-guanine phosphoribosyltransferase-deficient human diploid skin fibroblasts.
AB - Transport of purine bases and nucleosides by a variety of mammalian cell lines is generally accomplished by facilitated diffusion, a non-concentrative, saturable process. However, previous investigators have been unable to detect a saturable component for the transport of hypoxanthine by human fibroblasts deficient in hypoxanthine-guanine phosphoribosyltransferase, implying that in normal cells the enzyme actively participates in transport. In the present study we have used the phenomenon of countertransport to demonstrate the existence of a saturable transport mechanism in hypoxanthine-guanine phosphoribosyltransferase-deficient human diploid skin fibroblasts.
UR - http://www.scopus.com/inward/record.url?scp=0017689127&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017689127&partnerID=8YFLogxK
U2 - 10.1016/S0014-4827(77)80059-1
DO - 10.1016/S0014-4827(77)80059-1
M3 - Article
C2 - 891652
AN - SCOPUS:0017689127
SN - 0014-4827
VL - 108
SP - 461
EP - 464
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -