TY - JOUR
T1 - Transporting renal epithelium
T2 - Culture in hormonally defined serum-free medium
AU - Jefferson, Douglas M.
AU - Cobb, Melanie H.
AU - Gennaro, Joseph F.
AU - Scott, Walter N.
PY - 1980
Y1 - 1980
N2 - A hormonally defined medium was used to isolate a homogeneous epithelioid cell population from canine kidney. Monolayers of these cells form domes, an indication of active ion transport, and this process is inhibited by ouabain. This technique allows the isolation of primary cultures of renal epithelial cells, free of fibroblasts, for the characterization of biochemical and physiological properties related to renal function.
AB - A hormonally defined medium was used to isolate a homogeneous epithelioid cell population from canine kidney. Monolayers of these cells form domes, an indication of active ion transport, and this process is inhibited by ouabain. This technique allows the isolation of primary cultures of renal epithelial cells, free of fibroblasts, for the characterization of biochemical and physiological properties related to renal function.
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U2 - 10.1126/science.7434005
DO - 10.1126/science.7434005
M3 - Article
C2 - 7434005
AN - SCOPUS:0019174764
SN - 0036-8075
VL - 210
SP - 912
EP - 914
JO - Science
JF - Science
IS - 4472
ER -