Transporting renal epithelium

Culture in hormonally defined serum-free medium

Douglas M. Jefferson, Melanie H. Cobb, Joseph F. Gennaro, Walter N. Scott

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A hormonally defined medium was used to isolate a homogeneous epithelioid cell population from canine kidney. Monolayers of these cells form domes, an indication of active ion transport, and this process is inhibited by ouabain. This technique allows the isolation of primary cultures of renal epithelial cells, free of fibroblasts, for the characterization of biochemical and physiological properties related to renal function.

Original languageEnglish (US)
Pages (from-to)912-914
Number of pages3
JournalScience
Volume210
Issue number4472
StatePublished - 1980

Fingerprint

Serum-Free Culture Media
Epithelium
Kidney
Epithelioid Cells
Ion Transport
Ouabain
Canidae
Fibroblasts
Epithelial Cells
Population

ASJC Scopus subject areas

  • General

Cite this

Jefferson, D. M., Cobb, M. H., Gennaro, J. F., & Scott, W. N. (1980). Transporting renal epithelium: Culture in hormonally defined serum-free medium. Science, 210(4472), 912-914.

Transporting renal epithelium : Culture in hormonally defined serum-free medium. / Jefferson, Douglas M.; Cobb, Melanie H.; Gennaro, Joseph F.; Scott, Walter N.

In: Science, Vol. 210, No. 4472, 1980, p. 912-914.

Research output: Contribution to journalArticle

Jefferson, DM, Cobb, MH, Gennaro, JF & Scott, WN 1980, 'Transporting renal epithelium: Culture in hormonally defined serum-free medium', Science, vol. 210, no. 4472, pp. 912-914.
Jefferson DM, Cobb MH, Gennaro JF, Scott WN. Transporting renal epithelium: Culture in hormonally defined serum-free medium. Science. 1980;210(4472):912-914.
Jefferson, Douglas M. ; Cobb, Melanie H. ; Gennaro, Joseph F. ; Scott, Walter N. / Transporting renal epithelium : Culture in hormonally defined serum-free medium. In: Science. 1980 ; Vol. 210, No. 4472. pp. 912-914.
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