Transporting renal epithelium: Culture in hormonally defined serum-free medium

Douglas M. Jefferson, Melanie H. Cobb, Joseph F. Gennaro, Walter N. Scott

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A hormonally defined medium was used to isolate a homogeneous epithelioid cell population from canine kidney. Monolayers of these cells form domes, an indication of active ion transport, and this process is inhibited by ouabain. This technique allows the isolation of primary cultures of renal epithelial cells, free of fibroblasts, for the characterization of biochemical and physiological properties related to renal function.

Original languageEnglish (US)
Pages (from-to)912-914
Number of pages3
JournalScience
Volume210
Issue number4472
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • General

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