Transposition of an intron in yeast mitochondria requires a protein encoded by that intron

Ian G. Macreadie, Rose M. Scott, Andrew R. Zinn, Ronald A. Butow

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

The optional 1143 by intron in the yeast mitochondrial 21S rRNA gene (ω+) is nearly quantitatively inserted in genetic crosses into 21S rRNA alleles that lack it (ω-). The intron contains an open reading frame that can encode a protein of 235 amino acids, but no function has been ascribed to this sequence. We previously found an in vivo double-strand break in ω- DNA at or close to the intron insertion site only in zygotes of ω+ × ω- crosses that appears with the same kinetics as intron insertion. We now show that mutations in the intron open reading frame that would alter the translation product simultaneously inhibit nonreciprocal ω recombination and the in vivo double-strand break in ω- DNA. These results provide evidence that the open reading frame encodes a protein required for intron transposition and support the role of the double-strand break in the process.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalCell
Volume41
Issue number2
DOIs
StatePublished - Jun 1985

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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