Transposition of an intron in yeast mitochondria requires a protein encoded by that intron

Ian G. Macreadie, Rose M. Scott, Andrew R. Zinn, Ronald A. Butow

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

The optional 1143 by intron in the yeast mitochondrial 21S rRNA gene (ω+) is nearly quantitatively inserted in genetic crosses into 21S rRNA alleles that lack it (ω-). The intron contains an open reading frame that can encode a protein of 235 amino acids, but no function has been ascribed to this sequence. We previously found an in vivo double-strand break in ω- DNA at or close to the intron insertion site only in zygotes of ω+ × ω- crosses that appears with the same kinetics as intron insertion. We now show that mutations in the intron open reading frame that would alter the translation product simultaneously inhibit nonreciprocal ω recombination and the in vivo double-strand break in ω- DNA. These results provide evidence that the open reading frame encodes a protein required for intron transposition and support the role of the double-strand break in the process.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalCell
Volume41
Issue number2
DOIs
StatePublished - 1985

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Inteins
Mitochondria
Yeast
Introns
Yeasts
Open Reading Frames
Double-Stranded DNA Breaks
Proteins
Genetic Crosses
Zygote
rRNA Genes
DNA
Genetic Recombination
Alleles
Amino Acids
Genes
Mutation
Kinetics

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Transposition of an intron in yeast mitochondria requires a protein encoded by that intron. / Macreadie, Ian G.; Scott, Rose M.; Zinn, Andrew R.; Butow, Ronald A.

In: Cell, Vol. 41, No. 2, 1985, p. 395-402.

Research output: Contribution to journalArticle

Macreadie, Ian G. ; Scott, Rose M. ; Zinn, Andrew R. ; Butow, Ronald A. / Transposition of an intron in yeast mitochondria requires a protein encoded by that intron. In: Cell. 1985 ; Vol. 41, No. 2. pp. 395-402.
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