TY - JOUR
T1 - Transsarcolemmal calcium movements in arterially perfused rabbit right ventricle measured with extracellular calcium-sensitive dyes
AU - Hilgemann, D. W.
AU - Langer, G. A.
PY - 1984
Y1 - 1984
N2 - Activation-dependent changes of mean extracellular calcium concentrations were monitored spectrophotometrically in arterially perfused right ventricle of rabbit via extracellular application of the calcium-sensitive absorption dyes, antipyrylazo III and tetramethylmurexide. After rest periods of 3 minutes or longer, 10% of total dye-accessible calcium (300-500 μM) is depleted cumulatively from the extracellular space by four to eight beats at 2 Hz stimulation. During continued stimulation, or during quiescence following a depletion response, mean extracellular calcium concentrations return toward the prestimulatory level in the course of 2-4 minutes. A single stimulation placed 2-30 seconds subsequent to rapid pacing results in a potentiated beat with an accompanying net increment of mean extracellular calcium. Total dye-accessible calcium can increase by at least 4% at such a beat, and depletion responses can be repeated immediately thereafter. During a sequence of such responses, extracellular calcium lost cumulatively during three to five rapid beats is replenished, for the most part, by a single post-stimulatory beat. These results demonstrate that most of the 'activator pool' of cellular calcium can turn over to the extracellular space during a single contraction cycle in rabbit myocardium, and suggest that, in mammalian myocardium, diastolic calcium efflux may be quantitatively negligible in relation to efflux during excitation-contraction coupling.
AB - Activation-dependent changes of mean extracellular calcium concentrations were monitored spectrophotometrically in arterially perfused right ventricle of rabbit via extracellular application of the calcium-sensitive absorption dyes, antipyrylazo III and tetramethylmurexide. After rest periods of 3 minutes or longer, 10% of total dye-accessible calcium (300-500 μM) is depleted cumulatively from the extracellular space by four to eight beats at 2 Hz stimulation. During continued stimulation, or during quiescence following a depletion response, mean extracellular calcium concentrations return toward the prestimulatory level in the course of 2-4 minutes. A single stimulation placed 2-30 seconds subsequent to rapid pacing results in a potentiated beat with an accompanying net increment of mean extracellular calcium. Total dye-accessible calcium can increase by at least 4% at such a beat, and depletion responses can be repeated immediately thereafter. During a sequence of such responses, extracellular calcium lost cumulatively during three to five rapid beats is replenished, for the most part, by a single post-stimulatory beat. These results demonstrate that most of the 'activator pool' of cellular calcium can turn over to the extracellular space during a single contraction cycle in rabbit myocardium, and suggest that, in mammalian myocardium, diastolic calcium efflux may be quantitatively negligible in relation to efflux during excitation-contraction coupling.
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U2 - 10.1161/01.RES.54.4.461
DO - 10.1161/01.RES.54.4.461
M3 - Article
C2 - 6325038
AN - SCOPUS:0021333661
SN - 0009-7330
VL - 54
SP - 461
EP - 467
JO - Circulation research
JF - Circulation research
IS - 4
ER -