TY - JOUR
T1 - Triggering of cultured neoplastic mast cells by antibodies to the receptor for IgE
AU - Isersky, C.
AU - Taurog, J. D.
AU - Poy, G.
AU - Metzger, H.
PY - 1978/12/1
Y1 - 1978/12/1
N2 - Cell surface receptors for IgE were isolated from detergent lysates of iodinated, IgE-saturated, rat basophilic leukemia cells by precipitation with anti-IgE antibodies followed by chromatography at acid pH. The isolated material showed a single 125I-band (m.w. ~58,000) on gel electrophoresis in sodium dodecyl sulfate and was used to immunize a rabbit. The resulting antiserum was reacted with lysates of surface iodinated mouse or rat tumor mast cells. Analysis of the precipitates on (10%) gel electrophoresis revealed one major peak comprising >80% of the detectable counts and having an estimated m.w. of ~58,000. The antiserum reacted with detergent-solubilized and cell-bound receptors in the presence or absence of excess IgE; it also inhibited the binding of 125I-IgE. Cultured mouse mastocytoma cells never exposed to IgE released 3H-serotonin when incubated with F(ab')2, but not Fab' fragments of the antiserum, which had been rigorously freed of IgE and anti-IgE. The release was inhibited in the presence of excess IgE, was Ca++ dependent, and equaled 80% of the maximum obtained with IgE and anti-IgE. The authors conclude that aggregation of the receptors for IgE provides the critical signal for cell activation.
AB - Cell surface receptors for IgE were isolated from detergent lysates of iodinated, IgE-saturated, rat basophilic leukemia cells by precipitation with anti-IgE antibodies followed by chromatography at acid pH. The isolated material showed a single 125I-band (m.w. ~58,000) on gel electrophoresis in sodium dodecyl sulfate and was used to immunize a rabbit. The resulting antiserum was reacted with lysates of surface iodinated mouse or rat tumor mast cells. Analysis of the precipitates on (10%) gel electrophoresis revealed one major peak comprising >80% of the detectable counts and having an estimated m.w. of ~58,000. The antiserum reacted with detergent-solubilized and cell-bound receptors in the presence or absence of excess IgE; it also inhibited the binding of 125I-IgE. Cultured mouse mastocytoma cells never exposed to IgE released 3H-serotonin when incubated with F(ab')2, but not Fab' fragments of the antiserum, which had been rigorously freed of IgE and anti-IgE. The release was inhibited in the presence of excess IgE, was Ca++ dependent, and equaled 80% of the maximum obtained with IgE and anti-IgE. The authors conclude that aggregation of the receptors for IgE provides the critical signal for cell activation.
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M3 - Article
C2 - 308073
AN - SCOPUS:0018146982
SN - 0022-1767
VL - 121
SP - 549
EP - 558
JO - Journal of Immunology
JF - Journal of Immunology
IS - 2
ER -