Triggering of cultured neoplastic mast cells by antibodies to the receptor for IgE

C. Isersky, J. D. Taurog, G. Poy, H. Metzger

Research output: Contribution to journalArticle

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Abstract

Cell surface receptors for IgE were isolated from detergent lysates of iodinated, IgE-saturated, rat basophilic leukemia cells by precipitation with anti-IgE antibodies followed by chromatography at acid pH. The isolated material showed a single 125I-band (m.w. ~58,000) on gel electrophoresis in sodium dodecyl sulfate and was used to immunize a rabbit. The resulting antiserum was reacted with lysates of surface iodinated mouse or rat tumor mast cells. Analysis of the precipitates on (10%) gel electrophoresis revealed one major peak comprising >80% of the detectable counts and having an estimated m.w. of ~58,000. The antiserum reacted with detergent-solubilized and cell-bound receptors in the presence or absence of excess IgE; it also inhibited the binding of 125I-IgE. Cultured mouse mastocytoma cells never exposed to IgE released 3H-serotonin when incubated with F(ab')2, but not Fab' fragments of the antiserum, which had been rigorously freed of IgE and anti-IgE. The release was inhibited in the presence of excess IgE, was Ca++ dependent, and equaled 80% of the maximum obtained with IgE and anti-IgE. The authors conclude that aggregation of the receptors for IgE provides the critical signal for cell activation.

Original languageEnglish (US)
Pages (from-to)549-558
Number of pages10
JournalJournal of Immunology
Volume121
Issue number2
StatePublished - 1978

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Cultured Tumor Cells
IgE Receptors
Mast Cells
Immunoglobulin E
Antibodies
Immune Sera
Detergents
Electrophoresis
Gels
Receptor Aggregation
Mastocytoma
Immunoglobulin Fab Fragments
Cell Surface Receptors
Sodium Dodecyl Sulfate
Chromatography
Serotonin
Leukemia
Rabbits
Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Triggering of cultured neoplastic mast cells by antibodies to the receptor for IgE. / Isersky, C.; Taurog, J. D.; Poy, G.; Metzger, H.

In: Journal of Immunology, Vol. 121, No. 2, 1978, p. 549-558.

Research output: Contribution to journalArticle

Isersky, C. ; Taurog, J. D. ; Poy, G. ; Metzger, H. / Triggering of cultured neoplastic mast cells by antibodies to the receptor for IgE. In: Journal of Immunology. 1978 ; Vol. 121, No. 2. pp. 549-558.
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