Triglycerides in the human kidney cortex: Relationship with body size

Ion A Bobulescu, Yair Lotan, Jianning Zhang, Tara R. Rosenthal, John T. Rogers, Beverley A Huet, Khashayar Sakhaee, Orson W Moe

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Obesity is associated with increased risk for kidney disease and uric acid nephrolithiasis, but the pathophysiological mechanisms underpinning these associations are incompletely understood. Animal experiments have suggested that renal lipid accumulation and lipotoxicity may play a role, but whether lipid accumulation occurs in humans with increasing body mass index (BMI) is unknown. The association between obesity and abnormal triglyceride accumulation in non-adipose tissues (steatosis) has been described in the liver, heart, skeletal muscle and pancreas, but not in the human kidney. We used a quantitative biochemical assay to quantify triglyceride in normal kidney cortex samples from 54 patients undergoing nephrectomy for localized renal cell carcinoma. In subsets of the study population we evaluated the localization of lipid droplets by Oil Red O staining and measured 16 common ceramide species by mass spectrometry. There was a positive correlation between kidney cortex trigyceride content and BMI (Spearman R = 0.27, P = 0.04). Lipid droplets detectable by optical microscopy had a sporadic distribution but were generally more prevalent in individuals with higher BMI, with predominant localization in proximal tubule cells and to a lesser extent in glomeruli. Total ceramide content was inversely correlated with triglycerides. We postulate that obesity is associated with abnormal triglyceride accumulation (steatosis) in the human kidney. In turn, steatosis and lipotoxicity may contribute to the pathogenesis of obesity-associated kidney disease and nephrolithiasis.

Original languageEnglish (US)
Article numbere101285
JournalPLoS One
Volume9
Issue number8
DOIs
StatePublished - Aug 29 2014

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Kidney Cortex
Body Size
cortex
Triglycerides
body size
Obesity
triacylglycerols
kidneys
Lipids
obesity
Nephrolithiasis
Body Mass Index
Ceramides
Kidney Diseases
renal calculi
body mass index
Kidney
ceramides
lipids
kidney diseases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Bobulescu, I. A., Lotan, Y., Zhang, J., Rosenthal, T. R., Rogers, J. T., Huet, B. A., ... Moe, O. W. (2014). Triglycerides in the human kidney cortex: Relationship with body size. PLoS One, 9(8), [e101285]. https://doi.org/10.1371/journal.pone.0101285

Triglycerides in the human kidney cortex : Relationship with body size. / Bobulescu, Ion A; Lotan, Yair; Zhang, Jianning; Rosenthal, Tara R.; Rogers, John T.; Huet, Beverley A; Sakhaee, Khashayar; Moe, Orson W.

In: PLoS One, Vol. 9, No. 8, e101285, 29.08.2014.

Research output: Contribution to journalArticle

Bobulescu IA, Lotan Y, Zhang J, Rosenthal TR, Rogers JT, Huet BA et al. Triglycerides in the human kidney cortex: Relationship with body size. PLoS One. 2014 Aug 29;9(8). e101285. https://doi.org/10.1371/journal.pone.0101285
Bobulescu, Ion A ; Lotan, Yair ; Zhang, Jianning ; Rosenthal, Tara R. ; Rogers, John T. ; Huet, Beverley A ; Sakhaee, Khashayar ; Moe, Orson W. / Triglycerides in the human kidney cortex : Relationship with body size. In: PLoS One. 2014 ; Vol. 9, No. 8.
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