TRMT5 Mutations Cause a Defect in Post-transcriptional Modification of Mitochondrial tRNA Associated with Multiple Respiratory-Chain Deficiencies

Christopher A. Powell, Robert Kopajtich, Aaron R. D'Souza, Joanna Rorbach, Laura S. Kremer, Ralf A. Husain, Cristina Dallabona, Claudia Donnini, Charlotte L. Alston, Helen Griffin, Angela Pyle, Patrick F. Chinnery, Tim M. Strom, Thomas Meitinger, Richard J. Rodenburg, Gudrun Schottmann, Markus Schuelke, Nadine Romain, Ronald G. Haller, Ileana Ferrero & 4 others Tobias B. Haack, Robert W. Taylor, Holger Prokisch, Michal Minczuk

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Deficiencies in respiratory-chain complexes lead to a variety of clinical phenotypes resulting from inadequate energy production by the mitochondrial oxidative phosphorylation system. Defective expression of mtDNA-encoded genes, caused by mutations in either the mitochondrial or nuclear genome, represents a rapidly growing group of human disorders. By whole-exome sequencing, we identified two unrelated individuals carrying compound heterozygous variants in TRMT5 (tRNA methyltransferase 5). TRMT5 encodes a mitochondrial protein with strong homology to members of the class I-like methyltransferase superfamily. Both affected individuals presented with lactic acidosis and evidence of multiple mitochondrial respiratory-chain-complex deficiencies in skeletal muscle, although the clinical presentation of the two affected subjects was remarkably different; one presented in childhood with failure to thrive and hypertrophic cardiomyopathy, and the other was an adult with a life-long history of exercise intolerance. Mutations in TRMT5 were associated with the hypomodification of a guanosine residue at position 37 (G37) of mitochondrial tRNA; this hypomodification was particularly prominent in skeletal muscle. Deficiency of the G37 modification was also detected in human cells subjected to TRMT5 RNAi. The pathogenicity of the detected variants was further confirmed in a heterologous yeast model and by the rescue of the molecular phenotype after re-expression of wild-type TRMT5 cDNA in cells derived from the affected individuals. Our study highlights the importance of post-transcriptional modification of mitochondrial tRNAs for faithful mitochondrial function.

Original languageEnglish (US)
Article number1910
Pages (from-to)319-328
Number of pages10
JournalAmerican Journal of Human Genetics
Volume97
Issue number2
DOIs
StatePublished - Aug 6 2015

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tRNA Methyltransferases
Electron Transport
Transfer RNA
Mutation
Skeletal Muscle
Exome
Phenotype
Mitochondrial Diseases
Failure to Thrive
Lactic Acidosis
Molecular Models
Guanosine
Hypertrophic Cardiomyopathy
Mitochondrial Proteins
Oxidative Phosphorylation
Methyltransferases
RNA Interference
Mitochondrial DNA
Virulence
Complementary DNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

TRMT5 Mutations Cause a Defect in Post-transcriptional Modification of Mitochondrial tRNA Associated with Multiple Respiratory-Chain Deficiencies. / Powell, Christopher A.; Kopajtich, Robert; D'Souza, Aaron R.; Rorbach, Joanna; Kremer, Laura S.; Husain, Ralf A.; Dallabona, Cristina; Donnini, Claudia; Alston, Charlotte L.; Griffin, Helen; Pyle, Angela; Chinnery, Patrick F.; Strom, Tim M.; Meitinger, Thomas; Rodenburg, Richard J.; Schottmann, Gudrun; Schuelke, Markus; Romain, Nadine; Haller, Ronald G.; Ferrero, Ileana; Haack, Tobias B.; Taylor, Robert W.; Prokisch, Holger; Minczuk, Michal.

In: American Journal of Human Genetics, Vol. 97, No. 2, 1910, 06.08.2015, p. 319-328.

Research output: Contribution to journalArticle

Powell, CA, Kopajtich, R, D'Souza, AR, Rorbach, J, Kremer, LS, Husain, RA, Dallabona, C, Donnini, C, Alston, CL, Griffin, H, Pyle, A, Chinnery, PF, Strom, TM, Meitinger, T, Rodenburg, RJ, Schottmann, G, Schuelke, M, Romain, N, Haller, RG, Ferrero, I, Haack, TB, Taylor, RW, Prokisch, H & Minczuk, M 2015, 'TRMT5 Mutations Cause a Defect in Post-transcriptional Modification of Mitochondrial tRNA Associated with Multiple Respiratory-Chain Deficiencies', American Journal of Human Genetics, vol. 97, no. 2, 1910, pp. 319-328. https://doi.org/10.1016/j.ajhg.2015.06.011
Powell, Christopher A. ; Kopajtich, Robert ; D'Souza, Aaron R. ; Rorbach, Joanna ; Kremer, Laura S. ; Husain, Ralf A. ; Dallabona, Cristina ; Donnini, Claudia ; Alston, Charlotte L. ; Griffin, Helen ; Pyle, Angela ; Chinnery, Patrick F. ; Strom, Tim M. ; Meitinger, Thomas ; Rodenburg, Richard J. ; Schottmann, Gudrun ; Schuelke, Markus ; Romain, Nadine ; Haller, Ronald G. ; Ferrero, Ileana ; Haack, Tobias B. ; Taylor, Robert W. ; Prokisch, Holger ; Minczuk, Michal. / TRMT5 Mutations Cause a Defect in Post-transcriptional Modification of Mitochondrial tRNA Associated with Multiple Respiratory-Chain Deficiencies. In: American Journal of Human Genetics. 2015 ; Vol. 97, No. 2. pp. 319-328.
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AU - D'Souza, Aaron R.

AU - Rorbach, Joanna

AU - Kremer, Laura S.

AU - Husain, Ralf A.

AU - Dallabona, Cristina

AU - Donnini, Claudia

AU - Alston, Charlotte L.

AU - Griffin, Helen

AU - Pyle, Angela

AU - Chinnery, Patrick F.

AU - Strom, Tim M.

AU - Meitinger, Thomas

AU - Rodenburg, Richard J.

AU - Schottmann, Gudrun

AU - Schuelke, Markus

AU - Romain, Nadine

AU - Haller, Ronald G.

AU - Ferrero, Ileana

AU - Haack, Tobias B.

AU - Taylor, Robert W.

AU - Prokisch, Holger

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