Tropism for tuft cells determines immune promotion of norovirus pathogenesis

Craig B. Wilen, Sanghyun Lee, Leon L. Hsieh, Robert C. Orchard, Chandni Desai, Barry L. Hykes, Michael R. McAllaster, Dale R. Balce, Taylor Feehley, Jonathan R. Brestoff, Christina A. Hickey, Christine C. Yokoyama, Ya Ting Wang, Donna A. MacDuff, Darren Kreamalmayer, Michael R. Howitt, Jessica A. Neil, Ken Cadwell, Paul M. Allen, Scott A. HandleyMenno van Lookeren Campagne, Megan T. Baldridge, Herbert W. Virgin

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Complex interactions between host immunity and the microbiome regulate norovirus infection. However, the mechanism of host immune promotion of enteric virus infection remains obscure. The cellular tropism of noroviruses is also unknown. Recently, we identified CD300lf as a murine norovirus (MNoV) receptor. In this study, we have shown that tuft cells, a rare type of intestinal epithelial cell, express CD300lf and are the target cell for MNoV in the mouse intestine. We found that type 2 cytokines, which induce tuft cell proliferation, promote MNoV infection in vivo. These cytokines can replace the effect of commensal microbiota in promoting virus infection. Our work thus provides insight into how the immune system and microbes can coordinately promote enteric viral infection.

Original languageEnglish (US)
Pages (from-to)204-208
Number of pages5
JournalScience
Volume360
Issue number6385
DOIs
StatePublished - Apr 13 2018
Externally publishedYes

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Norovirus
Tropism
Microbiota
Virus Diseases
Enterovirus Infections
Cytokines
Infection
Intestines
Immune System
Immunity
Epithelial Cells
Cell Proliferation

ASJC Scopus subject areas

  • General

Cite this

Wilen, C. B., Lee, S., Hsieh, L. L., Orchard, R. C., Desai, C., Hykes, B. L., ... Virgin, H. W. (2018). Tropism for tuft cells determines immune promotion of norovirus pathogenesis. Science, 360(6385), 204-208. https://doi.org/10.1126/science.aar3799

Tropism for tuft cells determines immune promotion of norovirus pathogenesis. / Wilen, Craig B.; Lee, Sanghyun; Hsieh, Leon L.; Orchard, Robert C.; Desai, Chandni; Hykes, Barry L.; McAllaster, Michael R.; Balce, Dale R.; Feehley, Taylor; Brestoff, Jonathan R.; Hickey, Christina A.; Yokoyama, Christine C.; Wang, Ya Ting; MacDuff, Donna A.; Kreamalmayer, Darren; Howitt, Michael R.; Neil, Jessica A.; Cadwell, Ken; Allen, Paul M.; Handley, Scott A.; van Lookeren Campagne, Menno; Baldridge, Megan T.; Virgin, Herbert W.

In: Science, Vol. 360, No. 6385, 13.04.2018, p. 204-208.

Research output: Contribution to journalArticle

Wilen, CB, Lee, S, Hsieh, LL, Orchard, RC, Desai, C, Hykes, BL, McAllaster, MR, Balce, DR, Feehley, T, Brestoff, JR, Hickey, CA, Yokoyama, CC, Wang, YT, MacDuff, DA, Kreamalmayer, D, Howitt, MR, Neil, JA, Cadwell, K, Allen, PM, Handley, SA, van Lookeren Campagne, M, Baldridge, MT & Virgin, HW 2018, 'Tropism for tuft cells determines immune promotion of norovirus pathogenesis', Science, vol. 360, no. 6385, pp. 204-208. https://doi.org/10.1126/science.aar3799
Wilen, Craig B. ; Lee, Sanghyun ; Hsieh, Leon L. ; Orchard, Robert C. ; Desai, Chandni ; Hykes, Barry L. ; McAllaster, Michael R. ; Balce, Dale R. ; Feehley, Taylor ; Brestoff, Jonathan R. ; Hickey, Christina A. ; Yokoyama, Christine C. ; Wang, Ya Ting ; MacDuff, Donna A. ; Kreamalmayer, Darren ; Howitt, Michael R. ; Neil, Jessica A. ; Cadwell, Ken ; Allen, Paul M. ; Handley, Scott A. ; van Lookeren Campagne, Menno ; Baldridge, Megan T. ; Virgin, Herbert W. / Tropism for tuft cells determines immune promotion of norovirus pathogenesis. In: Science. 2018 ; Vol. 360, No. 6385. pp. 204-208.
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AU - Howitt, Michael R.

AU - Neil, Jessica A.

AU - Cadwell, Ken

AU - Allen, Paul M.

AU - Handley, Scott A.

AU - van Lookeren Campagne, Menno

AU - Baldridge, Megan T.

AU - Virgin, Herbert W.

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