Tryptophan hydroxylase is expressed by photoreceptors in Xenopus laevis retina

C. B. Green, G. M. Cahill, J. C. Besharse

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Serotonin has important roles, both as a neurotransmitter and as a precursor for melatonin synthesis. In the vertebrate retina, the role and the localization of serotonin have been controversial. Studies examining serotonin immunoreactivity and uptake of radiolabeled serotonin have localized serotonin to inner retinal neurons, particularly populations of amacrine cells, and have proposed that these cells are the sites of serotonin synthesis. However, other reports identify other cells, such as bipolars and photoreceptors, as serotonergic neurons. Tryptophan hydroxylase (TPH), the rate-limiting enzyme in the serotonin synthetic pathway, was recently cloned from Xenopus laevis retina, providing a specific probe for localization of serotonin synthesis. Here we demonstrate that the majority of retinal mRNA encoding TPH is present in photoreceptor cells in Xenopus laevis retina. These cells also contain TPH enzyme activity. Therefore, in addition to being the site of melatonin synthesis, the photoreceptor cells also synthesize serotonin, providing a supply of the substrate needed for the production of melatonin.

Original languageEnglish (US)
Pages (from-to)663-670
Number of pages8
JournalVisual Neuroscience
Volume12
Issue number4
DOIs
StatePublished - 1995

Keywords

  • Melatonin
  • Photoreceptor cells
  • Serotonin
  • Tryptophan hydroxylase

ASJC Scopus subject areas

  • Physiology
  • Sensory Systems

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