TTX-resistant ca2+ oscillation in cultured hypothalamus: Similarity to the mammalian circadian pacemaker

Shin Yamazaki, Shin Ichi T Inouye, Yoichiro Kuroda

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Dissociated cultures of rat hypothalamic cells showed spontaneous oscillations in intracellular Ca2+ with periods of 5-8 s (ultradian rhythm). Replacement of H2O by deuterium oxide in the culture medium lengthened the period of this oscillation, while tetrodotoxin did not inhibit the deuterium oxide sensitive Ca2+ oscillation. Since these characteristics resemble those of the mammalian circadian pacemaker, the ultradian Ca2+ rhythms may be involved in the circadian rhythm or have a similar rhythm generation mechanism. The gap junction inhibitor, halothane, stopped this oscillation. Gap junctions may be necessary in order to generate rhythmic oscillations.

Original languageEnglish (US)
Pages (from-to)1306-1308
Number of pages3
JournalNeuroReport
Volume6
Issue number9
DOIs
StatePublished - Jun 1995

Keywords

  • Calcium oscillation
  • Circadian rhythm
  • Deuterium oxide
  • Dissociated culture
  • Gap junctions
  • Hypothalamus
  • Ultradian rhythm

ASJC Scopus subject areas

  • Neuroscience(all)

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