Tubular membrane invaginations coated by dynamin rings are induced by GTP-γS in nerve terminals

Kohji Takei, Peter S. McPherson, Sandra L. Schmid, Pietro De Camilli

Research output: Contribution to journalArticle

616 Citations (Scopus)

Abstract

THE mechanisms through which synaptic vesicle membranes are reinternalized after exocytosis remain a matter of debate1-5. Because several vesicular transport steps require GTP hydrolysis6-9, GTP-γS may help identify intermediates in synaptic vesicle recycling. In GTP-γS-treated nerve terminals, we observed tubular invaginations of the plasmalemma that were often, but not always, capped by a clathrin-coated bud. Strikingly, the walls of these tubules were decorated by transverse electron-dense rings that were morphologically similar to structures formed by dynamin around tubular templates10,11. Dynamin is a GTPase implicated in synaptic vesicle endocytosis12-14 and here we show that the walls of these membranous tubules, but not their distal ends, were positive for dynamin immunoreactivity. These findings demonstrate that dynamin and clathrin act at different sites in the formation of endocytic vesicles. They strongly support a role for dynamin in the fission reaction and suggest that stabilization of the GTP-bound conformation of dynamin leads to tubule formation by progressive elongation of the vesicle stalk.

Original languageEnglish (US)
Pages (from-to)186-190
Number of pages5
JournalNature
Volume374
Issue number6518
StatePublished - 1995

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Dynamins
Guanosine Triphosphate
Membranes
Synaptic Vesicles
Clathrin
Transport Vesicles
Synaptic Membranes
GTP Phosphohydrolases
Exocytosis
Electrons

ASJC Scopus subject areas

  • General

Cite this

Takei, K., McPherson, P. S., Schmid, S. L., & Camilli, P. D. (1995). Tubular membrane invaginations coated by dynamin rings are induced by GTP-γS in nerve terminals. Nature, 374(6518), 186-190.

Tubular membrane invaginations coated by dynamin rings are induced by GTP-γS in nerve terminals. / Takei, Kohji; McPherson, Peter S.; Schmid, Sandra L.; Camilli, Pietro De.

In: Nature, Vol. 374, No. 6518, 1995, p. 186-190.

Research output: Contribution to journalArticle

Takei, K, McPherson, PS, Schmid, SL & Camilli, PD 1995, 'Tubular membrane invaginations coated by dynamin rings are induced by GTP-γS in nerve terminals', Nature, vol. 374, no. 6518, pp. 186-190.
Takei, Kohji ; McPherson, Peter S. ; Schmid, Sandra L. ; Camilli, Pietro De. / Tubular membrane invaginations coated by dynamin rings are induced by GTP-γS in nerve terminals. In: Nature. 1995 ; Vol. 374, No. 6518. pp. 186-190.
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