Tumor cell lysis by T cells distinct from NK cells and alloantigen-specific cytotoxic T cells

Dwain L Thiele, Peter E. Lipsky

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Abstract

The generation and mechanism of tumor cell lysis by cytotoxic T cells derived from natural killer cell (NK) and allospecific cytotoxic T cell (CTL)-depleted precursors were examined. NK cells and the precursors of alloantigen-specific CTL were deleted from human peripheral blood lymphocytes by preincubation with l-leucyl-l-leucine methyl ester (Leu-Leu-OMe). Following phytohemagglutinin activation, CD3(+), CD4(+) or CD8(+), CD11b(-), CD16(-), and NKH1(-) killer cells capable of lysing a broad spectrum of tumor targets were generated. Cytolysis was not strictly lectin dependent as similar killer cells were generated by activating Leu-Leu-OMe-treated T cells with immobilized monoclonal antibodies to the CD3 molecular complex. The rate of tumor cell lysis by these mitogen-activated T cells was slower than that mediated by CD3(-) NK cells. Tumor cell lysis by mitogen-activated killers was inhibited by anti-CD3 but was not restricted by major histocompatibility complex antigen expression on target cells or by CD4/CD8 expression on effectors. Although similar to NK cells in susceptibility to anti-LFA-1 inhibition of killing, these mitogen-activated killer cells were more sensitive to the inhibitory effects of anti-CD2 than were CD3(-)-activated NK-like cells. Thus, tumor cell lysis by CD3(+) cytotoxic cells generated from Leu-Leu-OMe-treated lymphocytes appears to be mediated in part by mechanisms distinct from those employed by CD3(-) NK cells or antigen-specific CTL.

Original languageEnglish (US)
Pages (from-to)405-423
Number of pages19
JournalClinical Immunology and Immunopathology
Volume49
Issue number3
DOIs
StatePublished - 1988

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Isoantigens
Natural Killer Cells
T-Lymphocytes
Neoplasms
Mitogens
Lymphocytes
T-Lymphoid Precursor Cells
Immobilized Antibodies
CD3 Antigens
Lymphocyte Function-Associated Antigen-1
Histocompatibility Antigens
Phytohemagglutinins
Major Histocompatibility Complex
Lectins

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Tumor cell lysis by T cells distinct from NK cells and alloantigen-specific cytotoxic T cells. / Thiele, Dwain L; Lipsky, Peter E.

In: Clinical Immunology and Immunopathology, Vol. 49, No. 3, 1988, p. 405-423.

Research output: Contribution to journalArticle

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