Tumor dormancy and cell signaling: Anti-μ-induced apoptosis in human B-lymphoma cells is not caused by an APO-1-APO-1 ligand interaction

Emilian Racila, Robert Hsueh, Radu Marches, Thomas F. Tucker, Peter H. Krammer, Richard H. Scheuermann, Jonathan W. Uhr

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Signal transduction initiated by crosslinking of antigen-specific receptors on T- and B-lymphoma cells induces apoptosis. In T-lymphoma cells, such crosslinking results in upregulation of the APO-1 ligand, which then interacts with induced or constitutively expressed APO-1, thereby triggering apoptosis. Here we show that crosslinking the membrane imniunoglobulin on human lymphoma cells (Daudi) (that constitutively express APO-1) does not induce synthesis of APO-1 ligand. Further, a noncytotoxic fragment of anti-APO-1 antibody that blocks T-cell-receptor-mediated apoptosis in T-lymphoma cells does not block anti-μ-induced apoptosis. Hence, in B-lymphoma cells, apoptosis induced by signaling via membrane IgM is not mediated by the APO-1 ligand.

Original languageEnglish (US)
Pages (from-to)2165-2168
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number5
DOIs
StatePublished - Mar 5 1996

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Keywords

  • Apoptosis
  • B-cell lymphoma
  • Membrane immunoglobulin

ASJC Scopus subject areas

  • General

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