Tumor oximetry: Demonstration of an enhanced dynamic mapping procedure using fluorine-19 echo planar magnetic resonance imaging in the Dunning prostate R3327-AT1 rat tumor

S. Hunjan, D. Zhao, A. Constantinescu, E. W. Hahn, P. P. Antich, R. P. Mason

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Purpose: We have developed an enhanced approach to measuring regional oxygen tension (pO2) dynamics in tumors. The technique is demonstrated in a group of 8 Dunning prostate rat tumors (R3327-AT1) with respect to respiratory challenge. Methods and Materials: Hexafluorobenzene was injected directly into the tumors of anesthetized rats. 19F nuclear magnetic resonance echo planar imaging relaxometry was performed to obtain maps of regional tumor oxygenation under baseline conditions and when the inhaled gas was changed to oxygen or carbogen. Results: Sequential pO2 maps required 8 min, with a typical precision of 1-3 torr at 30-100 individual regions across a tumor. When rats breathed 33% oxygen, distinct heterogeneity was observed for baseline oxygenation in each tumor with pO2 values ranging from hypoxic to greater than 100 torr. Larger tumors showed significantly lower baseline pO2. Respiratory challenge with oxygen or carbogen produced significant increases in tumor oxygenation with a close correlation between the response to each gas at individual locations. Regions of both small and large tumors responded to respiratory challenge, but the rate was generally much faster in initially well-oxygenated regions. Conclusions: Regional pO2 was assessed quantitatively and the response of multiple individual tumor regions observed simultaneously with respect to interventions.

Original languageEnglish (US)
Pages (from-to)1097-1108
Number of pages12
JournalInternational Journal of Radiation Oncology Biology Physics
Volume49
Issue number4
DOIs
StatePublished - Mar 15 2001

Fingerprint

oximetry
Echo-Planar Imaging
Oximetry
rats
fluorine
magnetic resonance
Prostate
echoes
tumors
Neoplasms
oxygenation
Oxygen
oxygen
Gases
oxygen tension
Fluorine-19 Magnetic Resonance Imaging
Respiratory Rate
gases
Magnetic Resonance Spectroscopy

Keywords

  • Carbogen
  • Fluorine magnetic resonance imaging
  • Hypoxia
  • Oxygen
  • Tumor

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Tumor oximetry : Demonstration of an enhanced dynamic mapping procedure using fluorine-19 echo planar magnetic resonance imaging in the Dunning prostate R3327-AT1 rat tumor. / Hunjan, S.; Zhao, D.; Constantinescu, A.; Hahn, E. W.; Antich, P. P.; Mason, R. P.

In: International Journal of Radiation Oncology Biology Physics, Vol. 49, No. 4, 15.03.2001, p. 1097-1108.

Research output: Contribution to journalArticle

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abstract = "Purpose: We have developed an enhanced approach to measuring regional oxygen tension (pO2) dynamics in tumors. The technique is demonstrated in a group of 8 Dunning prostate rat tumors (R3327-AT1) with respect to respiratory challenge. Methods and Materials: Hexafluorobenzene was injected directly into the tumors of anesthetized rats. 19F nuclear magnetic resonance echo planar imaging relaxometry was performed to obtain maps of regional tumor oxygenation under baseline conditions and when the inhaled gas was changed to oxygen or carbogen. Results: Sequential pO2 maps required 8 min, with a typical precision of 1-3 torr at 30-100 individual regions across a tumor. When rats breathed 33{\%} oxygen, distinct heterogeneity was observed for baseline oxygenation in each tumor with pO2 values ranging from hypoxic to greater than 100 torr. Larger tumors showed significantly lower baseline pO2. Respiratory challenge with oxygen or carbogen produced significant increases in tumor oxygenation with a close correlation between the response to each gas at individual locations. Regions of both small and large tumors responded to respiratory challenge, but the rate was generally much faster in initially well-oxygenated regions. Conclusions: Regional pO2 was assessed quantitatively and the response of multiple individual tumor regions observed simultaneously with respect to interventions.",
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