Tumor oxygen dynamics: Correlation of in vivo MRI with histological findings

Dawen Zhao, Sophia Ran, Anca Constantinescu, Eric W. Hahn, Ralph P. Mason

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Tumor oxygenation has long been recognized as a significant factor influencing cancer therapy. We recently established a novel magnetic resonance in vivo approach to measuring regional tumor oxygen tension, FREDOM (Fluorocarbon Relaxometry Using Echo Planar Imaging for Dynamic Oxygen Mapping), using hexafluorobenzene (HFB) as the reporter molecule. We have now investigated oxygen dynamics in the two Dunning prostate R3327 rat tumor sublines, AT1 and H. FREDOM revealed considerable intratumoral heterogeneity in the distribution of pO2 values in both sublines. The anaplastic faster-growing AT1 tumors were more hypoxic compared with the size-matched, well-differentiated, and slower-growing H tumors. Respiratory challenge with oxygen produced significant increases in mean and median pO2 in all the H tumors (P<.001), but no response in half of the larger AT1 tumors (>3 cm3). Immunohistochemical studies using the hypoxia marker, pimonidazole, and the vascular endothelial cell marker, CD31, confirmed that the H tumors had more extensive vasculature and less hypoxia than the AT1 tumors. These results further validate the utilization of FREDOM to monitor tumor oxygenation and concur with the hypothesis that the level of hypoxia is related to tumor growth rate and poor vascularity.

Original languageEnglish (US)
Pages (from-to)308-318
Number of pages11
JournalNeoplasia
Volume5
Issue number4
StatePublished - Jul 1 2003

    Fingerprint

Keywords

  • F nuclear magnetic resonance (NMR)
  • Hypoxia
  • Immunohistochemistry
  • Oxygen tension
  • Prostate tumor

ASJC Scopus subject areas

  • Cancer Research

Cite this

Zhao, D., Ran, S., Constantinescu, A., Hahn, E. W., & Mason, R. P. (2003). Tumor oxygen dynamics: Correlation of in vivo MRI with histological findings. Neoplasia, 5(4), 308-318.